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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450787
Gene symbol: Dkk3
Gene name: dickkopf WNT signaling pathway inhibitor 3
Probe: UI-M-AL0-abx-a-08-0-UI.s1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen S8k: embryonic day 11.5 (more )
Note: Double labeled: blue - Dkk3; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S8_k Expression is present in a dorsal domain that encompasses the eminentia thalami, prethalamus and the paraventricular hypothalamic nucleus.
Specimen S8l: embryonic day 11.5 (more )
Note: Double labeled: blue - Dkk3; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S8_l Expression is present in a dorsal domain that encompasses the eminentia thalami, prethalamus and the paraventricular hypothalamic nucleus.
Specimen S8m: embryonic day 11.5 (more )
Note: Double labeled: blue - Dkk3; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S8_m Expression is present in a dorsal domain that encompasses the eminentia thalami, prethalamus and the paraventricular hypothalamic nucleus.
Specimen S8n: embryonic day 11.5 (more )
Note: Double labeled: blue - Dkk3; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S8_n Expression is present in a dorsal domain that encompasses the eminentia thalami, prethalamus and the paraventricular hypothalamic nucleus.
Specimen Dkk3 - E11.5: embryonic day 11.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Ambiguous Dkk3-Shh-E11.5-sagittal , Dkk3-Shh E11.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer Strong Non-Uniform Dkk3-Shh E11.5-coronal , Dkk3-Shh-E11.5-sagittal There is strong staining in the VZ of the prethalamus, PVH nucleus & the AVH. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, dorsal & ventral POAs, VMH & ARH nuclei, anterior & posterior IDs, premam, mam & supramam zones & TT.
TS19: telencephalon mantle layer Ambiguous Dkk3-Shh E11.5-coronal , Dkk3-Shh-E11.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Strong Non-Uniform Dkk3-Shh-E11.5-sagittal , Dkk3-Shh E11.5-coronal There is strong staining in the ventricular zone of the cerebral cortex and weak staining in the ganglionic eminences.
Specimen Dkk3 - E12.5: embryonic day 12.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous Dkk3-SHH-E12.5-sagittal , Dkk3-Shh E12.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Strong Dkk3-SHH-E12.5-sagittal , Dkk3-Shh E12.5-coronal There is strong staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous Dkk3-Shh E12.5-coronal , Dkk3-SHH-E12.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Ambiguous Dkk3-Shh E12.5-coronal , Dkk3-SHH-E12.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer Ambiguous Dkk3-SHH-E12.5-sagittal , Dkk3-Shh E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Ambiguous Dkk3-SHH-E12.5-sagittal , Dkk3-Shh E12.5-coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer Ambiguous Dkk3-Shh E12.5-coronal , Dkk3-SHH-E12.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Very strong Non-Uniform Dkk3-SHH-E12.5-sagittal , Dkk3-Shh E12.5-coronal There is very strong staining in the ventricular zone of the PVH nucleus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer Ambiguous Dkk3-Shh E12.5-coronal , Dkk3-SHH-E12.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer Very strong Non-Uniform Dkk3-Shh E12.5-coronal , Dkk3-SHH-E12.5-sagittal There is very strong staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.
Specimen Dkk3 - E14.5: embryonic day 14.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Ambiguous Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Strong Dkk3-SHH-E14.5-sagittal , Dkk3-Shh E14.5-coronal There is strong staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate Ambiguous Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Ambiguous Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer Very strong Non-Uniform Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal There is very strong staining in the ventricular zone of the premam zone and the TT. Staining is either absent or ubiquitous in the ventricular zone of the mam and supramam zones.
TS22: ganglionic eminence Ambiguous Dkk3-SHH-E14.5-sagittal , Dkk3-Shh E14.5-coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer Ambiguous Dkk3-SHH-E14.5-sagittal , Dkk3-Shh E14.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH , VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Very strong Non-Uniform Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal There is very strong staining in the ventricular zone of the dorsal and ventral POAs, the PVH and VMH nuclei, the AVH and anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the ARH nucleus.
TS22: thalamus mantle layer Ambiguous Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer Very strong Dkk3-SHH-E14.5-sagittal , Dkk3-Shh E14.5-coronal There is very strong staining in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Dkk3 - E16.5: embryonic day 16.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Very strong Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal There is very strong staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Very strong Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal There is very strong staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate Very strong Dkk3-Shh E16.5-coronal , Dkk3-SHH-E16.5-sagittal There is very strong staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Ambiguous Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer Very strong Non-Uniform Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal There is very strong staining in the ventricular zone of the premam zone. Staining is either absent or ubiquitous in the ventricular zone of the mam and supramam zones.
TS24: ganglionic eminence Moderate Non-Uniform Dkk3-Shh E16.5-coronal , Dkk3-SHH-E16.5-sagittal There is moderate staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS24: hypothalamus mantle layer Ambiguous Dkk3-Shh E16.5-coronal , Dkk3-SHH-E16.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer Very strong Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal There is very strong staining in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Ambiguous Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer Very strong Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal There is very strong staining in the ventricular zone of the dorsal thalamus and prethalamus.
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