Probe:	UI-M-BH3-ath-g-10-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	4c	Expression is present in the supramammillary nucleus.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	4d	Expression is present in the supramammillary nucleus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S4_a	Expresssion is present in the supramammillary nucleus.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	S4_b	Expresssion is present in the supramammillary nucleus.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	S4_c	Expresssion is present in the supramammillary nucleus.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	S4_d	Expresssion is present in the supramammillary nucleus.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	S6_a	Expression is present in the supramammillary nucleus.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	S6_b	Expression is present in the supramammillary nucleus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Very strong	Non-Uniform	Irx5-Shh E11.5-sagittal , Irx5-Shh E11.5-coronal	Staining is very strong in the MZ of the supramam zone & strong in dorsal thalamus. Staining is either absent or ubiquitous in the MZ of the prethalamus, dorsal & ventral POAs, PVH, VMH & ARH nuclei, AVH, anterior & posterior IDs, premam & mam zones & TT.
TS19: diencephalon lateral wall ventricular layer	Very strong	Non-Uniform	Irx5-Shh E11.5-sagittal , Irx5-Shh E11.5-coronal	Staining is very strong in the VZ of the supramam zone & weak in dorsal thalamus. Staining is either absent or ubiquitous in the VZ of the prethalamus, dorsal & ventral POAs, PVH, VMH & ARH nuclei, AVH, anterior & posterior IDs, premam & mam zones & TT.
TS19: telencephalon mantle layer	Ambiguous		Irx5-Shh E11.5-coronal , Irx5-Shh E11.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Ambiguous		Irx5-Shh E11.5-coronal , Irx5-Shh E11.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Irx5-Shh E12.5-sagittal , Irx5-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Irx5-Shh E12.5-sagittal , Irx5-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Irx5-Shh E12.5-coronal , Irx5-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Very strong	Non-Uniform	Irx5-Shh E12.5-coronal , Irx5-Shh E12.5-sagittal	There is very strong staining in the mantle zone of the supramam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and mam zones and the TT.
TS20: diencephalon ventricular layer	Very strong	Non-Uniform	Irx5-Shh E12.5-sagittal , Irx5-Shh E12.5-coronal	There is very strong staining in the ventricular zone of the supramam zone. Staining is either absent or ubiquitous in the ventricular zone of the premam and mam zones and the TT.
TS20: ganglionic eminence	Ambiguous		Irx5-Shh E12.5-coronal , Irx5-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer	Ambiguous		Irx5-Shh E12.5-sagittal , Irx5-Shh E12.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Ambiguous		Irx5-Shh E12.5-sagittal , Irx5-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer	Very strong	Non-Uniform	Irx5-Shh E12.5-sagittal , Irx5-Shh E12.5-coronal	There is very strong staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus.
TS20: thalamus ventricular layer	Weak	Non-Uniform	Irx5-Shh E12.5-coronal , Irx5-Shh E12.5-sagittal	There is weak staining in the ventricular zone of the dorsal thalamus. Staining is either absent or ubiquitous in the ventricular zone of the prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Irx5-Shh E14.5-coronal , Irx5-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Irx5-Shh E14.5-sagittal , Irx5-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Irx5-Shh E14.5-sagittal , Irx5-Shh E14.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Very strong	Non-Uniform	Irx5-Shh E14.5-sagittal , Irx5-Shh E14.5-coronal	There is very strong staining in the mantle zone of the supramam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and mam zones and the TT.
TS22: diencephalon ventricular layer	Ambiguous		Irx5-Shh E14.5-sagittal , Irx5-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Ambiguous		Irx5-Shh E14.5-coronal , Irx5-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer	Ambiguous		Irx5-Shh E14.5-sagittal , Irx5-Shh E14.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer	Ambiguous		Irx5-Shh E14.5-coronal , Irx5-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer	Strong	Non-Uniform	Irx5-Shh E14.5-coronal , Irx5-Shh E14.5-sagittal	There is strong staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus.
TS22: thalamus ventricular layer	Weak	Non-Uniform	Irx5-Shh E14.5-coronal , Irx5-Shh E14.5-sagittal	There is weak staining in the ventricular zone of the dorsal thalamus. Staining is either absent or ubiquitous in the ventricular zone of the prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Irx5-Shh E16.5-coronal , Irx5-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		Irx5-Shh E16.5-sagittal , Irx5-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Irx5-Shh E16.5-coronal , Irx5-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Very strong	Non-Uniform	Irx5-Shh E16.5-sagittal , Irx5-Shh E16.5-coronal	There is very strong staining in the mantle zone of the supramam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and mam zones.
TS24: diencephalon ventricular layer	Ambiguous		Irx5-Shh E16.5-sagittal , Irx5-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence	Ambiguous		Irx5-Shh E16.5-coronal , Irx5-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer	Ambiguous		Irx5-Shh E16.5-sagittal , Irx5-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer	Ambiguous		Irx5-Shh E16.5-sagittal , Irx5-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer	Strong	Non-Uniform	Irx5-Shh E16.5-coronal , Irx5-Shh E16.5-sagittal	There is strong staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS24: thalamus ventricular layer	Moderate	Non-Uniform	Irx5-Shh E16.5-coronal , Irx5-Shh E16.5-sagittal	There is moderate staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.