Probe:	UI-M-BH3-asu-c-05-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present		3k	Expression is present in the prethalamus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S5_e	Expression is present in the prethalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	S5_f	Expression is present in the prethalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present	Regionally restricted	S5_g	Expression is present in the prethalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS24: thalamus	Present	Regionally restricted	S5_h	Expression is present in the prethalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	S6_m	Expression is present in the prethalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	S6_n	Expression is present in the prethalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Ambiguous		Gsh2-Shh E11.5-sagittal , Gsh2-Shh E11.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer	Very strong	Non-Uniform	Gsh2-Shh E11.5-sagittal , Gsh2-Shh E11.5-coronal	There is very strong staining in the VZ of the prethalamus. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, dorsal & ventral POAs, the PVH, VMH & ARH nuclei, the AVH, anterior & posterior IDs, premam, mam & supramam zones & TT.
TS19: telencephalon mantle layer	Ambiguous		Gsh2-Shh E11.5-coronal , Gsh2-Shh E11.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Very strong	Non-Uniform	Gsh2-Shh E11.5-coronal , Gsh2-Shh E11.5-sagittal	There is very strong staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Gsh2-Shh E12.5-sagittal , Gsh2-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Gsh2-Shh E12.5-sagittal , Gsh2-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Gsh2-Shh E12.5-sagittal , Gsh2-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Ambiguous		Gsh2-Shh E12.5-sagittal , Gsh2-Shh E12.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer	Ambiguous		Gsh2-Shh E12.5-coronal , Gsh2-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence	Very strong	Non-Uniform	Gsh2-Shh E12.5-coronal , Gsh2-Shh E12.5-sagittal	There is very strong staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS20: hypothalamus mantle layer	Ambiguous		Gsh2-Shh E12.5-sagittal , Gsh2-Shh E12.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Weak	Non-Uniform	Gsh2-Shh E12.5-sagittal , Gsh2-Shh E12.5-coronal	There is weak staining in the ventricular zone of the dorsal POA. Staining is either absent or ubiquitous in the ventricular zone of the ventral POA, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer	Ambiguous		Gsh2-Shh E12.5-coronal , Gsh2-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Very strong	Non-Uniform	Gsh2-Shh E12.5-coronal , Gsh2-Shh E12.5-sagittal	There is very strong staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Gsh2-Shh E14.5-coronal , Gsh2-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Gsh2-Shh E14.5-sagittal , Gsh2-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Gsh2-Shh E14.5-sagittal , Gsh2-Shh E14.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Ambiguous		Gsh2-Shh E14.5-sagittal , Gsh2-Shh E14.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Ambiguous		Gsh2-Shh E14.5-sagittal , Gsh2-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Very strong	Non-Uniform	Gsh2-Shh E14.5-coronal , Gsh2-Shh E14.5-sagittal	There is very strong staining in the ventricular zone of the ganglionic eminences and moderate, scattered staining in the mantle zone of the ganglionic eminences.
TS22: hypothalamus mantle layer	Moderate	Non-Uniform	Gsh2-Shh E14.5-coronal , Gsh2-Shh E14.5-sagittal	There is moderate staining in the mantle zone of the dorsal POA. Staining is either absent or ubiquitous in the mantle zone of the ventral POA, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer	Ambiguous		Gsh2-Shh E14.5-coronal , Gsh2-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer	Ambiguous		Gsh2-Shh E14.5-coronal , Gsh2-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Moderate	Non-Uniform	Gsh2-Shh E14.5-sagittal , Gsh2-Shh E14.5-coronal	There is moderate staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Gsh2-Shh E16.5-coronal , Gsh2-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		Gsh2-Shh E16.5-sagittal , Gsh2-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Gsh2-Shh E16.5-coronal , Gsh2-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Ambiguous		Gsh2-Shh E16.5-coronal , Gsh2-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer	Ambiguous		Gsh2-Shh E16.5-coronal , Gsh2-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence	Very strong	Non-Uniform	Gsh2-Shh E16.5-coronal , Gsh2-Shh E16.5-sagittal	There is very strong staining in the ventricular zone of the ganglionic eminences and moderate, scattered staining in the mantle zone of the ganglionic eminences.
TS24: hypothalamus mantle layer	Ambiguous		Gsh2-Shh E16.5-coronal , Gsh2-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer	Ambiguous		Gsh2-Shh E16.5-sagittal , Gsh2-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer	Ambiguous		Gsh2-Shh E16.5-sagittal , Gsh2-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Moderate	Non-Uniform	Gsh2-Shh E16.5-coronal , Gsh2-Shh E16.5-sagittal	There is moderate staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.