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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450873
Gene symbol: Lef1
Gene name: lymphoid enhancer binding factor 1
Probe: MGC clone 47927
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4e: embryonic day 12.5 (more )
Note: Double labeled: blue - Lef1; brown - Lhx6 (assay MGI:4452075).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted 4e Expression is present in the premammillary region.
Specimen 4f: embryonic day 16.5 (more )
Note: Double labeled: blue - Lef1; brown - Lhx6 (assay MGI:4452075).
Structure Level Pattern Image Note
TS24: diencephalon Present Regionally restricted 4f Expression is present in the premammillary region.
Specimen S4i: embryonic day 11.5 (more )
Note: Double labeled: blue - Lef1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S4_i Expression is present in the premammillary nucleus.
Specimen S4j: embryonic day 12.5 (more )
Note: Double labeled: blue - Lef1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted S4_j Expression is present in the premammillary nucleus.
Specimen S4k: embryonic day 14.5 (more )
Note: Double labeled: blue - Lef1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS22: diencephalon Present Regionally restricted S4_k Expression is present in the premammillary nucleus.
Specimen S4l: embryonic day 16.5 (more )
Note: Double labeled: blue - Lef1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS24: diencephalon Present Regionally restricted S4_l Expression is present in the premammillary nucleus.
Specimen S6c: embryonic day 12.5 (more )
Note: Double labeled: blue - Lef1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted S6_c Expression is present in the premammillary nucleus.
Specimen S6d: embryonic day 12.5; Shhtm2Amc/Shhtm2Amc, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant. Double labeled: blue - Lef1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted S6_d Expression in the mutant is markedly reduced compared to wild type. It is present in the premammillary nucleus but is restricted to the ventral midline.
Specimen Lef1 - E11.5: embryonic day 11.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Strong Non-Uniform LEF1(D)+SHH(F)-E11.5-CORONAL , LEF1(D)+SHH(F)-E11.5-SAGITTAL There is strong staining in the MZ of the dorsal thalamus, prethalamus & premam zone. Staining is either absent or ubiquitous in the MZ of the dorsal & ventral POAs, the PVH, VMH & ARH nuclei, the AVH, anterior & posterior IDs, mam & supramam zones & TT.
TS19: diencephalon lateral wall ventricular layer Weak Non-Uniform LEF1(D)+SHH(F)-E11.5-SAGITTAL , LEF1(D)+SHH(F)-E11.5-CORONAL There is weak staining in the VZ of the dorsal thalamus & prethalamus. Staining is either absent or ubiquitous in the VZ of the dorsal & ventral POAs, the PVH, VMH & ARH nuclei, the AVH, anterior & posterior IDs, the premam, mam & supramam zones & the TT.
TS19: telencephalon mantle layer Ambiguous LEF1(D)+SHH(F)-E11.5-SAGITTAL , LEF1(D)+SHH(F)-E11.5-CORONAL Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Strong Non-Uniform LEF1(D)+SHH(F)-E11.5-CORONAL , LEF1(D)+SHH(F)-E11.5-SAGITTAL There is strong staining in the ventricular zone of the cerebral cortex. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
Specimen Lef1 - E12.5: embryonic day 12.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous LEF1(D)+SHH(F)-E12.5-CORONAL , LEF1(D)+SHH(F)-E12.5-Sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Strong LEF1(D)+SHH(F)-E12.5-Sagittal , LEF1(D)+SHH(F)-E12.5-CORONAL There is strong staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous LEF1(D)+SHH(F)-E12.5-CORONAL , LEF1(D)+SHH(F)-E12.5-Sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Strong Non-Uniform LEF1(D)+SHH(F)-E12.5-Sagittal , LEF1(D)+SHH(F)-E12.5-CORONAL There is strong staining in the mantle zone of the premam zone. Staining is either absent or ubiquitous in the mantle zone of the mam and supramam zones and the TT.
TS20: diencephalon ventricular layer Ambiguous LEF1(D)+SHH(F)-E12.5-CORONAL , LEF1(D)+SHH(F)-E12.5-Sagittal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Ambiguous LEF1(D)+SHH(F)-E12.5-Sagittal , LEF1(D)+SHH(F)-E12.5-CORONAL Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer Ambiguous LEF1(D)+SHH(F)-E12.5-Sagittal , LEF1(D)+SHH(F)-E12.5-CORONAL Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Ambiguous LEF1(D)+SHH(F)-E12.5-Sagittal , LEF1(D)+SHH(F)-E12.5-CORONAL Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer Strong Non-Uniform LEF1(D)+SHH(F)-E12.5-CORONAL , LEF1(D)+SHH(F)-E12.5-Sagittal There is strong staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus.
TS20: thalamus ventricular layer Moderate Non-Uniform LEF1(D)+SHH(F)-E12.5-Sagittal , LEF1(D)+SHH(F)-E12.5-CORONAL There is moderate staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.
Specimen Lef1 - E14.5: embryonic day 14.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Ambiguous LEF1(D)+SHH(F)-E14.5-CORONAL , LEF1(D)+SHH(F)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Very strong LEF1(D)+SHH(F)-E14.5-CORONAL , LEF1(D)+SHH(F)-E14.5-SAGITTAL There is very strong staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate Ambiguous LEF1(D)+SHH(F)-E14.5-CORONAL , LEF1(D)+SHH(F)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Strong Non-Uniform LEF1(D)+SHH(F)-E14.5-SAGITTAL , LEF1(D)+SHH(F)-E14.5-CORONAL There is strong staining in the mantle zone of the premam zone. Staining is either absent or ubiquitous in the mantle zone of the TT and mam and supramam zones.
TS22: diencephalon ventricular layer Ambiguous LEF1(D)+SHH(F)-E14.5-CORONAL , LEF1(D)+SHH(F)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence Ambiguous LEF1(D)+SHH(F)-E14.5-CORONAL , LEF1(D)+SHH(F)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer Ambiguous LEF1(D)+SHH(F)-E14.5-SAGITTAL , LEF1(D)+SHH(F)-E14.5-CORONAL Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Ambiguous LEF1(D)+SHH(F)-E14.5-SAGITTAL , LEF1(D)+SHH(F)-E14.5-CORONAL Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer Ambiguous LEF1(D)+SHH(F)-E14.5-SAGITTAL , LEF1(D)+SHH(F)-E14.5-CORONAL Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer Ambiguous LEF1(D)+SHH(F)-E14.5-SAGITTAL , LEF1(D)+SHH(F)-E14.5-CORONAL Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Lef1 - E16.5: embryonic day 16.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Ambiguous LEF1(D)+SHH(F)-E16.5-SAGITTAL , LEF1(D)+SHH(F)-E16.5-CORONAL Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Moderate LEF1(D)+SHH(F)-E16.5-SAGITTAL , LEF1(D)+SHH(F)-E16.5-CORONAL There is moderate staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate Ambiguous LEF1(D)+SHH(F)-E16.5-CORONAL , LEF1(D)+SHH(F)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Strong Non-Uniform LEF1(D)+SHH(F)-E16.5-SAGITTAL , LEF1(D)+SHH(F)-E16.5-CORONAL There is strong staining in the mantle zone of the premam zone. Staining is either absent or ubiquitous in the mantle zone of the mam and supramam zones.
TS24: diencephalon ventricular layer Ambiguous LEF1(D)+SHH(F)-E16.5-SAGITTAL , LEF1(D)+SHH(F)-E16.5-CORONAL Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence Ambiguous LEF1(D)+SHH(F)-E16.5-CORONAL , LEF1(D)+SHH(F)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer Ambiguous LEF1(D)+SHH(F)-E16.5-CORONAL , LEF1(D)+SHH(F)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer Ambiguous LEF1(D)+SHH(F)-E16.5-SAGITTAL , LEF1(D)+SHH(F)-E16.5-CORONAL Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Very strong Non-Uniform LEF1(D)+SHH(F)-E16.5-CORONAL , LEF1(D)+SHH(F)-E16.5-SAGITTAL There is very strong staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus
TS24: thalamus ventricular layer Ambiguous LEF1(D)+SHH(F)-E16.5-CORONAL , LEF1(D)+SHH(F)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
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11/19/2024
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