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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450886
Gene symbol: Pomc
Gene name: pro-opiomelanocortin-alpha
Probe: UI-M-AJ1-agz-c-01-0-UI.s1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen S4q: embryonic day 11.5 (more )
Note: Double labeled: blue - Pomc; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S4_q Expression is present in the arcuate nucleus.
Specimen S4r: embryonic day 12.5 (more )
Note: Double labeled: blue - Pomc; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted S4_r Expression is present in the arcuate nucleus.
Specimen S4s: embryonic day 14.5 (more )
Note: Double labeled: blue - Pomc; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS22: hypothalamus Present Regionally restricted S4_s Expression is present in the arcuate nucleus.
Specimen S4t: embryonic day 16.5 (more )
Note: Double labeled: blue - Pomc; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS24: hypothalamus Present Regionally restricted S4_t Expression is present in the arcuate nucleus.
Specimen S6g: embryonic day 12.5 (more )
Note: Double labeled: blue - Pomc; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted S6_g Expression is present in the arcuate nucleus.
Specimen S6h: embryonic day 12.5; Shhtm2Amc/Shhtm2Amc, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant. Double labeled: blue - Pomc; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: hypothalamus Absent S6_h Arcuate nucleus-specific expression is absent from the mutant.
Specimen Pomc1 - E11.5: embryonic day 11.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Very strong Non-Uniform POMC(D)+SHH(D)-E11.5-SAGITTAL , POMC(D)+SHH(D)-E11.5-coronal Staining is very strong in the MZ of the ARH nucleus. Staining is either absent or ubiquitous in the MZ of the dorsal thalamus, prethalamus, dorsal & ventral POAs, PVH & VMH nuclei, AVH, anterior & posterior IDs, premam, mam & supramam zones & TT.
TS19: diencephalon lateral wall ventricular layer Ambiguous POMC(D)+SHH(D)-E11.5-SAGITTAL , POMC(D)+SHH(D)-E11.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer Ambiguous POMC(D)+SHH(D)-E11.5-SAGITTAL , POMC(D)+SHH(D)-E11.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Ambiguous POMC(D)+SHH(D)-E11.5-SAGITTAL , POMC(D)+SHH(D)-E11.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.
Specimen Pomc1 - E12.5: embryonic day 12.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous POMC(D)+SHH(D)-E12.5-SAGITTAL-1 , POMC(D)+SHH(D)-E12.5-SAGITTAL-2 , POMC(D)+SHH(D)-E12.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Ambiguous POMC(D)+SHH(D)-E12.5-SAGITTAL-1 , POMC(D)+SHH(D)-E12.5-coronal , POMC(D)+SHH(D)-E12.5-SAGITTAL-2 Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous POMC(D)+SHH(D)-E12.5-coronal , POMC(D)+SHH(D)-E12.5-SAGITTAL-1 , POMC(D)+SHH(D)-E12.5-SAGITTAL-2 Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Ambiguous POMC(D)+SHH(D)-E12.5-SAGITTAL-1 , POMC(D)+SHH(D)-E12.5-SAGITTAL-2 , POMC(D)+SHH(D)-E12.5-coronal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer Ambiguous POMC(D)+SHH(D)-E12.5-coronal , POMC(D)+SHH(D)-E12.5-SAGITTAL-1 , POMC(D)+SHH(D)-E12.5-SAGITTAL-2 Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Ambiguous POMC(D)+SHH(D)-E12.5-SAGITTAL-2 , POMC(D)+SHH(D)-E12.5-SAGITTAL-1 , POMC(D)+SHH(D)-E12.5-coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer Very strong Non-Uniform POMC(D)+SHH(D)-E12.5-coronal , POMC(D)+SHH(D)-E12.5-SAGITTAL-1 , POMC(D)+SHH(D)-E12.5-SAGITTAL-2 There is very strong staining in the mantle zone of the ARH nucleus. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH and VMH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Ambiguous POMC(D)+SHH(D)-E12.5-coronal , POMC(D)+SHH(D)-E12.5-SAGITTAL-2 , POMC(D)+SHH(D)-E12.5-SAGITTAL-1 Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer Ambiguous POMC(D)+SHH(D)-E12.5-SAGITTAL-1 , POMC(D)+SHH(D)-E12.5-coronal , POMC(D)+SHH(D)-E12.5-SAGITTAL-2 Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer Ambiguous POMC(D)+SHH(D)-E12.5-coronal , POMC(D)+SHH(D)-E12.5-SAGITTAL-1 , POMC(D)+SHH(D)-E12.5-SAGITTAL-2 Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Pomc1 - E14.5: embryonic day 14.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Ambiguous POMC(D)+SHH(D)-E14.5-coronal , POMC(D)+SHH(D)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Ambiguous POMC(D)+SHH(D)-E14.5-coronal , POMC(D)+SHH(D)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate Ambiguous POMC(D)+SHH(D)-E14.5-coronal , POMC(D)+SHH(D)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Ambiguous POMC(D)+SHH(D)-E14.5-coronal , POMC(D)+SHH(D)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer Ambiguous POMC(D)+SHH(D)-E14.5-SAGITTAL , POMC(D)+SHH(D)-E14.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence Ambiguous POMC(D)+SHH(D)-E14.5-SAGITTAL , POMC(D)+SHH(D)-E14.5-coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer Very strong Non-Uniform POMC(D)+SHH(D)-E14.5-SAGITTAL , POMC(D)+SHH(D)-E14.5-coronal There is very strong staining in the mantle zone of the ARH nucleus. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH and VMH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Ambiguous POMC(D)+SHH(D)-E14.5-coronal , POMC(D)+SHH(D)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer Ambiguous POMC(D)+SHH(D)-E14.5-SAGITTAL , POMC(D)+SHH(D)-E14.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer Ambiguous POMC(D)+SHH(D)-E14.5-coronal , POMC(D)+SHH(D)-E14.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Pomc1 - E16.5: embryonic day 16.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Ambiguous POMC(D)+SHH(D)-E16.5-SAGITTAL , POMC(D)+SHH(D)-E16.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Ambiguous POMC(D)+SHH(D)-E16.5-SAGITTAL , POMC(D)+SHH(D)-E16.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate Ambiguous POMC(D)+SHH(D)-E16.5-coronal , POMC(D)+SHH(D)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Ambiguous POMC(D)+SHH(D)-E16.5-coronal , POMC(D)+SHH(D)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer Ambiguous POMC(D)+SHH(D)-E16.5-SAGITTAL , POMC(D)+SHH(D)-E16.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence Ambiguous POMC(D)+SHH(D)-E16.5-coronal , POMC(D)+SHH(D)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer Very strong Non-Uniform POMC(D)+SHH(D)-E16.5-coronal , POMC(D)+SHH(D)-E16.5-SAGITTAL There is very strong staining in the mantle zone of the ARH nucleus. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH and VMH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer Ambiguous POMC(D)+SHH(D)-E16.5-coronal , POMC(D)+SHH(D)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Ambiguous POMC(D)+SHH(D)-E16.5-coronal , POMC(D)+SHH(D)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer Ambiguous POMC(D)+SHH(D)-E16.5-coronal , POMC(D)+SHH(D)-E16.5-SAGITTAL Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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11/19/2024
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