Gene Expression Data
Assay Details
Assay
|
Reference:
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J:157819
Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4452112
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Gene symbol:
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Nr5a1
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Gene name:
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nuclear receptor subfamily 5, group A, member 1
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Probe:
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IMAGE clone 6852029
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
|
|
Results
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Specimen
4g:
embryonic day 12.5
(more )
Note: Double labeled: blue - Nr5a1; brown - Lhx6 (assay MGI:4452075).
Specimen
4g:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
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Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Nr5a1; brown - Lhx6 (assay MGI:4452075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hypothalamus |
Present |
Regionally restricted |
4g
|
Expression is present in the ventromedial hypothalamic nucleus. It is not present in the intrahypothalamic diagonal or tuberomammillary terminal.
|
Specimen
4h:
embryonic day 16.5
(more )
Note: Double labeled: blue - Nr5a1; brown - Lhx6 (assay MGI:4452075).
Specimen
4h:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Nr5a1; brown - Lhx6 (assay MGI:4452075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: hypothalamus |
Present |
Regionally restricted |
4h
|
Expression is present in the ventromedial hypothalamic nucleus.
|
Specimen
S4u:
embryonic day 11.5
(more )
Note: Double labeled: blue - Nr5a1; brown - Shh (assay MGI:4452154).
Specimen
S4u:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Nr5a1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Present |
Regionally restricted |
S4_u
|
Expression is present in the ventromedial hypothalamic nucleus.
|
Specimen
S4v:
embryonic day 12.5
(more )
Note: Double labeled: blue - Nr5a1; brown - Shh (assay MGI:4452154).
Specimen
S4v:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Nr5a1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hypothalamus |
Present |
Regionally restricted |
S4_v
|
Expression is present in the ventromedial hypothalamic nucleus.
|
Specimen
S4w:
embryonic day 14.5
(more )
Note: Double labeled: blue - Nr5a1; brown - Shh (assay MGI:4452154).
Specimen
S4w:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Nr5a1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hypothalamus |
Present |
Regionally restricted |
S4_w
|
Expression is present in the ventromedial hypothalamic nucleus.
|
Specimen
S4x:
embryonic day 16.5
(more )
Note: Double labeled: blue - Nr5a1; brown - Shh (assay MGI:4452154).
Specimen
S4x:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Nr5a1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: hypothalamus |
Present |
Regionally restricted |
S4_x
|
Expression is present in the ventromedial hypothalamic nucleus.
|
Specimen
S6e:
embryonic day 12.5
(more )
Note: Double labeled: blue - Nr5a1; brown - Shh (assay MGI:4452154).
Specimen
S6e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Nr5a1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hypothalamus |
Present |
Regionally restricted |
S6_e
|
Expression is present in the ventromedial hypothalamic nucleus.
|
Specimen
S6f:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
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Shhtm2Amc/Shhtm2Amc Tg(Nkx2-1-cre)2Sand/0
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: blue - Nr5a1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hypothalamus |
Absent |
|
S6_f
|
Ventromedial hypothalamic nucleus-specific expression is absent from the mutant.
|
|