Pax6 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:103820 Reiter JF, et al., Tectonic, a novel regulator of the Hedgehog pathway required for both activation and inhibition. Genes Dev. 2006 Jan 1;20(1):22-7
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:4461989
Gene symbol:	Pax6
Gene name:	paired box 6

Antibody:

anti-Pax6 (#PRB-278P; #30130, #901301)

Results

3B wild type ©

Specimen 3B wild type: embryonic day 9.5 (more

)
Note: Double labeled: red - Pax6; green - Foxa2 (assay MGI:4462006).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3B wild type

3B Tectonic-/- ©

Specimen 3B Tectonic-/-: embryonic day 9.5; Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Foxa2 (assay MGI:4462006).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3B Tectonic-/-	Expression was expanded compared to wild type.

3B Ptch-/- ©

Specimen 3B Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: Double labeled: red - Pax6; green - Foxa2 (assay MGI:4462006).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak		3B Ptch-/-

3B Tectonic-/- Ptch-/- ©

Specimen 3B Tectonic-/- Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps, Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Foxa2 (assay MGI:4462006).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3B Tectonic-/- Ptch-/-	Expression was expanded compared to wild type.

3C wild type ©

Specimen 3C wild type: embryonic day 9.5 (more

)
Note: Double labeled: red - Pax6; green - Nkx2-2 (assay MGI:4462009).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3C wild type

3C Tectonic-/- ©

Specimen 3C Tectonic-/-: embryonic day 9.5; Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Nkx2-2 (assay MGI:4462009).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3C Tectonic-/-	Expression was expanded compared to wild type.

3C Ptch-/- ©

Specimen 3C Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: Double labeled: red - Pax6; green - Nkx2-2 (assay MGI:4462009).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak		3C Ptch-/-

3C Tectonic-/- Ptch-/- ©

Specimen 3C Tectonic-/- Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps, Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Nkx2-2 (assay MGI:4462009).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3C Tectonic-/- Ptch-/-	Expression was expanded compared to wild type.

3D wild type ©

Specimen 3D wild type: embryonic day 9.5 (more

)
Note: Double labeled: red - Pax6; green - Isl1 (assay MGI:4462015) and Isl2 (assay MGI:4462016).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3D wild type

3D Tectonic-/- ©

Specimen 3D Tectonic-/-: embryonic day 9.5; Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Isl1 (assay MGI:4462015) and Isl2 (assay MGI:4462016).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3D Tectonic-/-	Expression was expanded compared to wild type.

3D Ptch-/- ©

Specimen 3D Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: Double labeled: red - Pax6; green - Isl1 (assay MGI:4462015) and Isl2 (assay MGI:4462016).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak		3D Ptch-/-

3D Tectonic-/- Ptch-/- ©

Specimen 3D Tectonic-/- Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps, Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Isl1 (assay MGI:4462015) and Isl2 (assay MGI:4462016).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3D Tectonic-/- Ptch-/-	Expression was expanded compared to wild type.

3F wild type ©

Specimen 3F wild type: embryonic day 10.5 (more

)
Note: Double labeled: red - Pax6; green - Pax3 (assay MGI:4462013).

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3F wild type	Expression was detected dorsally.

Specimen 3F Tectonic-/-: embryonic day 10.5; Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Pax3 (assay MGI:4462013).

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3F Tectonic-/-	Expression was expanded compared to wild type.

Specimen 3F Rab23 opb/opb: embryonic day 10.5; Rab23^opb2/Rab23^opb2 (more

)
Note: Double labeled: red - Pax6; green - Pax3 (assay MGI:4462013).

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3F Rab23 opb/opb	Expression was shifted dorsally compared to wild type.

Specimen 3F Tect-/- Rab23 opb/opb: embryonic day 10.5; Rab23^opb2/Rab23^opb2, Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Pax3 (assay MGI:4462013).

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3F Tect-/- Rab23 opb/opb	Expression was not shifted dorsally compared to wild type.

S4_wild type ©

Specimen S4 wild type: embryonic day 11.5 (more

)
Note: Double labeled: red - Pax6; green - Msx1 (assay MGI:4462020) and Msx2 (assay MGI:4462027).

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		S4_wild type

Specimen S4 Tectonic-/-: embryonic day 11.5; Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Msx1 (assay MGI:4462020) and Msx2 (assay MGI:4462027).

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		S4_Tectonic-/-

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24