Hsd3b3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:153498 Diez-Roux G, et al., A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol. 2011;9(1):e1000582
Assay type:	RNA in situ
MGI Accession ID:	MGI:4825466
Gene symbol:	Hsd3b3
Gene name:	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 3

Probe:	MGC clone 107584
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing. A tyramide-biotin/streptavidin amplification step was included in the in situ hybridization procedure.

Results

euxassay_005913_04 ©

Specimen euxassay_005913_04: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: testis medullary region	Strong	Regionally restricted	euxassay_005913_04

euxassay_005913_05 ©

Specimen euxassay_005913_05: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Strong	Regionally restricted	euxassay_005913_05
TS23: testis medullary region	Strong	Regionally restricted	euxassay_005913_05

euxassay_005913_06 ©

Specimen euxassay_005913_06: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Strong	Regionally restricted	euxassay_005913_06
TS23: testis medullary region	Strong	Regionally restricted	euxassay_005913_06

euxassay_005913_07 ©

Specimen euxassay_005913_07: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Strong	Regionally restricted	euxassay_005913_07
TS23: testis medullary region	Strong	Regionally restricted	euxassay_005913_07

euxassay_005913_08 ©

Specimen euxassay_005913_08: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Strong	Regionally restricted	euxassay_005913_08

euxassay_005913_13 ©

Specimen euxassay_005913_13: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Strong	Regionally restricted	euxassay_005913_13

euxassay_005913_14 ©

Specimen euxassay_005913_14: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Strong	Regionally restricted	euxassay_005913_14

euxassay_005913_15 ©

Specimen euxassay_005913_15: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Strong	Regionally restricted	euxassay_005913_15

Specimen euxassay_005913_16: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Strong	Regionally restricted	euxassay_005913_16

Specimen euxassay_005913_17: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: testis medullary region	Strong	Regionally restricted	euxassay_005913_17

Specimen euxassay_005913_18: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: testis medullary region	Strong	Regionally restricted	euxassay_005913_18

Specimen euxassay_005913_19: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: testis medullary region	Strong	Regionally restricted	euxassay_005913_19

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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