Gene Expression Data
Assay Details
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Assay
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Reference:
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J:104961
St Germain C, et al., Expression and transient nuclear translocation of proprotein convertase 1 (PC1) during mouse preimplantation embryonic development. Mol Reprod Dev. 2005 Dec;72(4):483-93
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4947705
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Gene symbol:
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Pcsk1
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Gene name:
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proprotein convertase subtilisin/kexin type 1
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Antibody:
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anti-PC1-NT
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Assay notes:
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The antibody used in this assay recognizes all isoforms of the protein.
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Results
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Specimen
1C:
embryonic day 0.5
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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CF-1
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Specimen
2C:
embryonic day 1.0
(more )
Specimen
2C:
(close )
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Genetic Background:
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CF-1
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Non-Uniform |
3Bb
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Expression is unevenly distributed and relatively more intense in the cytoplasm.
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Specimen
BL:
embryonic day 3.5
(more )
Specimen
BL:
(close )
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Genetic Background:
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CF-1
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS5: inner cell mass |
Present |
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3Bc
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In blastocysts expression appears to be concentrated in the trophectoderm and inner cell mass.
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| TS5: trophectoderm |
Present |
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3Bc
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In blastocysts expression appears to be concentrated in the trophectoderm and inner cell mass.
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Specimen
4A:
embryonic day 3.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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CF-1
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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4Af
,
4Ad
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There is a substantial amount of non-cytoplasmic staining.
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Specimen
4B:
embryonic day 3.5
(more )
Specimen
4B:
(close )
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Genetic Background:
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CF-1
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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4Bf
,
4Bd
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Expression is nuclear in a number of blastomeres.
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Analysis Tools