Gene Expression Data
Assay Details
Assay
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Reference:
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J:108338
Tanifuji-Terai N, et al., Expression of keratin 12 and maturation of corneal epithelium during development and postnatal growth. Invest Ophthalmol Vis Sci. 2006 Feb;47(2):545-51
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5141139
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Gene symbol:
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Krt12
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Gene name:
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keratin 12
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Antibody:
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anti-K12N
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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The authors used two antibodies. One was specific for the amino terminus and the other for the carboxyl terminus. Identical results were obtained with each.
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Results
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Specimen
E15.5:
embryonic day 15.5
(more )
Specimen
E15.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: cornea epithelium |
Present |
Regionally restricted |
1_K12 E15.5
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Expression is present in the suprabasal epithelial cells.
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Specimen
E17.5:
embryonic day 17.5
(more )
Specimen
E17.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS25: cornea epithelium |
Present |
Regionally restricted |
1_K12 E17.5
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Expression is present in the suprabasal epithelial cells. This is the time of peak expression.
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Specimen
E19.5:
embryonic day 19.5
(more )
Specimen
E19.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 19.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: cornea epithelium |
Present |
|
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Expression levels are beginning to decline in the suprabasal epithelial cells.
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Specimen
P0:
postnatal day 0
(more )
Specimen
P0:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
P2:
postnatal day 2
(more )
Specimen
P2:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
P4:
postnatal day 4
(more )
Specimen
P4:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: cornea epithelium |
Present |
Regionally restricted |
1_K12 P4
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There is no expression in the corneal basal cells.
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Specimen
P10:
postnatal day 10
(more )
Specimen
P10:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: cornea epithelium |
Present |
Non-Uniform |
1_K12 P10
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Expression is primarily limited to suprabasal and superficial layers.
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Specimen
P30:
postnatal day 30
(more )
Specimen
P30:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 30
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: cornea epithelium |
Present |
Non-Uniform |
2_P30
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A significant number of basal corneal epithelial cells do not stain, although the number that do stain increases as the animals age.
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Specimen
P90:
postnatal day 90
(more )
Specimen
P90:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 90
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cornea epithelium |
Present |
Non-Uniform |
2_P90
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A significant number of basal corneal epithelial cells do not stain, although the number that do stain increases as the animals age.
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