Probe:	Foxp2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: diencephalon mantle layer	Present	Regionally restricted	4F	There is broad expression in the posterior half of the prosomere 2 mantle region.
TS20: diencephalon ventricular layer	Present	Regionally restricted	4F	There is expression in some of the prosomere 2 ventricular zone but not in the anterior p2 VZ.

Structure	Level	Pattern	Image	Note
TS20: diencephalon mantle layer	Present	Regionally restricted	4G	There is broad expression in the posterior half of the prosomere 2 mantle region but not in the anterior p2 mantle region.
TS20: diencephalon ventricular layer	Present	Regionally restricted	4G	There is expression in some of the prosomere 2 ventricular zone but not in the anterior p2 VZ.
TS20: diencephalon	Weak	Regionally restricted	4G	Expression is present in the postmitotic region of prosomere 3.

Structure	Level	Pattern	Image	Note
TS20: diencephalon mantle layer	Present	Regionally restricted	4H	There is broad expression in the posterior half of the prosomere 2 mantle region but not in the anterior p2 mantle region.
TS20: diencephalon ventricular layer	Present	Regionally restricted	4H	There is expression in some of the prosomere 2 ventricular zone but not in the anterior p2 VZ.
TS20: diencephalon	Weak	Regionally restricted	4H	Expression is present in the postmitotic region of prosomere 3.

Structure	Level	Pattern	Image	Note
TS20: diencephalon mantle layer	Present	Regionally restricted	4I	There is broad expression in the posterior half of the prosomere 2 mantle region but not in the anterior p2 mantle region.
TS20: diencephalon ventricular layer	Present	Regionally restricted	4I	There is expression in some of the prosomere 2 ventricular zone but not in the anterior p2 VZ.
TS20: diencephalon	Weak	Regionally restricted	4I	Expression is present in the postmitotic region of prosomere 3.

Structure	Level	Pattern	Image	Note
TS20: diencephalon mantle layer	Present	Regionally restricted	4J	There is broad expression in the posterior half of the prosomere 2 mantle region.
TS20: diencephalon ventricular layer	Present	Regionally restricted	4J	There is expression in some of the prosomere 2 ventricular zone but not in the anterior p2 VZ.

Structure	Level	Pattern	Image	Note
TS23: thalamus	Present	Widespread	7I	Expression is present throughout prosomere 2.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Strong	Regionally restricted	8F	There is strong expression in the posterior part of the hypothalamic primordium; there is no expression in prosomere 2.