Probe:	Lef-1 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS21: saccule mesenchyme	Present			Expression is present in the mesenchymal cells of the inner ear around the cochlea.
TS21: spinal cord meninges	Absent
TS21: rib cartilage condensation	Absent
TS21: thoracic vertebral cartilage condensation	Absent
TS21: tail vertebral cartilage condensation	Present
TS21: lung mesenchyme	Present			Expression is present in the mesenchymal cells around the bronchioles.
TS21: lung epithelium	Absent			There is no expression in bronchiolar epithelial cells.
TS21: basioccipital pre-cartilage condensation	Absent
TS21: palatal shelf	Absent			There is no expression in the palate.

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Present		2C
TS22: tooth	Present	Regionally restricted	2C	Expression is present in the odontoblasts.
TS22: hindlimb digit	Present	Regionally restricted	2C	Expression is present at the apex.
TS22: choroid plexus	Weak		2C
TS22: lung	Present		2C
TS22: mesenchyme of urogenital sinus	Present		2C
TS22: paramesonephric duct of male	Present	Regionally restricted	2C	Expression is present in the mesenchymal cells.
TS22: genital tubercle	Present	Diffuse	2C	Expression is observed throughout the genital eminence.
TS22: urethra	Present		2C	Expression is present around the epithelial cells of the developing urethra.
TS22: tail vertebral pre-cartilage condensation	Present		2C	Expression is present in tail prevertebrae.
TS22: hip	Present	Regionally restricted	2C	Expression is present in osteogenic cells.
TS22: pituitary gland	Present		2C
TS22: lower lip	Strong		2C
TS22: upper lip	Strong		2C
TS22: midbrain	Present	Regionally restricted	2C	Expression is present in the roof.
TS22: thalamus	Present	Regionally restricted	2C	Expression is present in the dorsal thalamus.
TS22: hypothalamus	Present		2C
TS22: telencephalon	Present	Regionally restricted	2C	Expression is present in a part of the telencephalon.
TS22: mesenchyme of metanephros	Strong		2C	Expression is present in the mesenchymal cells surrounding the metanephros.
TS22: adrenal gland	Absent		2C
TS22: mandible	Absent		2C
TS22: maxilla	Absent		2C
TS22: rib cartilage condensation	Absent		2C
TS22: thoracic vertebral cartilage condensation	Absent		2C	There is no expression in the thoracic prevertebrae.
TS22: palatal shelf	Absent		2C	There is no expression in the palate.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Graded	3B	Expression levels are higher in the mesoderm than the ectoderm. It is present in an anterioposterior gradient, with the highest levels being expressed posteriorly.
TS11: embryo mesoderm	Present	Graded	3B	Expression levels are higher in the mesoderm than the ectoderm. It is present in an anterioposterior gradient, with the highest levels being expressed posteriorly.
TS11: amnion	Present		3B
TS11: chorion	Present		3B
TS11: embryo endoderm	Absent		3B
TS11: Reichert's membrane	Absent		3B
TS11: trophectoderm	Absent		3B	There is no expression in trophectoderm-derived cells.

Structure	Level	Pattern	Image	Note
TS11: allantois	Present

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		4B
TS15: trunk mesenchyme	Present	Regionally restricted	4B	Expression is present in part of the lateral plate mesoderm.
TS15: 1st branchial arch	Present		4B	It is likely expression occurs in neural crest-derived cells.
TS15: 2nd branchial arch	Present		4B	It is likely expression occurs in neural crest-derived cells.
TS15: trunk mesenchyme derived from neural crest	Present		4B	Expression is present in mesenchymal cells that most likely derive from neural crest.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		5D	It is likely expression occurs in neural crest-derived cells.
TS17: 2nd branchial arch	Present		5D	It is likely expression occurs in neural crest-derived cells.
TS17: 3rd branchial arch	Present		5D	It is likely expression occurs in neural crest-derived cells.
TS17: 1st branchial arch ectoderm	Present		5D	Expression is present in the epithelial lining between the mandibular and maxillary component.
TS17: 3rd branchial arch ectoderm	Present		5D	Expression is present in the epithelium.
TS17: oral region cavity	Present	Regionally restricted	5D	Expression is present in the roof.

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present		6C	Expression is present in the mesenchymal cells around the bronchioles.
TS22: lung epithelium	Absent		6C	There is no expression in bronchiolar epithelial cells.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Strong		7B
TS19: midbrain	Strong		7B
TS19: telencephalon	Strong		7B
TS19: hindbrain	Absent		7B
TS19: mandibular process	Present		7B
TS19: latero-nasal process mesenchyme	Present		7B
TS19: medial-nasal process mesenchyme	Present		7B

Structure	Level	Pattern	Image	Note
TS22: saccule mesenchyme	Present		8	Expression is present in the mesenchymal cells of the inner ear around the cochlea.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present			Expression is present in the progress zone, between the apical ectodermal ridge and the marginal vein.
TS17: embryo	Present	Regionally restricted		Expression is present in cells clustered on either side of the neural tube, suggesting a neural crest origin.
TS17: mesonephros mesenchyme	Weak			Expression is present in mesenchymal cells surrounding the mesonephric tubules.
TS17: lung mesenchyme	Present			Expression is present in the mesenchymal cells around the bronchioles.

Structure	Level	Pattern	Image	Note
TS27: lung	Absent
TS27: renal medullary interstitium	Trace			Minimal amounts of expression are occasionally detected in medullary mesenchymal cells.
TS27: brain	Absent
TS27: hemolymphoid system	Present			Expression is present in lymphoid tissue.