Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79856
Rallu M, et al., Dorsoventral patterning is established in the telencephalon of mutants lacking both Gli3 and Hedgehog signaling. Development. 2002 Nov;129(21):4963-74
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5298525
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Gene symbol:
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Nkx2-1
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Gene name:
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NK2 homeobox 1
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Probe:
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Nkx2.1 probe10
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
Not shown:
embryonic day 10.5
(more  )
Specimen
Not shown:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
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Expression is present in the medial ganglionic eminence.
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Not shown:
embryonic day 12.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4F:
embryonic day 12.5
(more )
Specimen
4F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: medial ganglionic eminence |
Present |
Regionally restricted |
4F
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Expression is present in the most ventral part of the telencephalon (the medial ganglionic eminence).
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Specimen
4G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3+
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
4G
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A small region of expression is observed at the ventral midline.
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Specimen
4H:
embryonic day 12.5
(more )
Note: Overlay of images of adjacent sections; red - Nkx2-1; green - Dlx2.
Specimen
4H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Overlay of images of adjacent sections; red - Nkx2-1; green - Dlx2.
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Specimen
4I:
embryonic day 12.5; Gli3Xt-J/Gli3 +, Shhtm1Chg/ Shhtm1Chg
(more )
Note: Overlay of images of adjacent sections; red - Nkx2-1; green - Dlx2.
Specimen
4I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3+
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Overlay of images of adjacent sections; red - Nkx2-1; green - Dlx2.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
4I
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A small region of expression is observed at the ventral midline.
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Specimen
5E:
embryonic day 10.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
5E
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Expression is present in the medial ganglionic eminence.
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
5F
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Expression in the mutant is similar to that seen for wild type.
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Specimen
5G:
embryonic day 10.5
(more )
Note: Overlay of images of adjacent sections; red - Nkx2-1; green - Dlx2.
Specimen
5G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Overlay of images of adjacent sections; red - Nkx2-1; green - Dlx2.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
5G
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Expression is present in the medial ganglionic eminence.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Overlay of images of adjacent sections; red - Nkx2-1; green - Dlx2.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
5H
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Expression in the mutant is similar to that seen for wild type.
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Specimen
5M:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C3HeB/FeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Smotm1Amc/Smotm1Amc
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
5M
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Despite the exencephaly seen in the mutant, expression is still present in the ventral telencephalon in a pattern reminiscent of that seen in wild type.
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Analysis Tools