Gene Expression Data
Assay Details
Assay
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Reference:
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J:56368
Jean D, et al., Six6 (Optx2) is a novel murine Six3-related homeobox gene that demarcates the presumptive pituitary/hypothalamic axis and the ventral optic stalk. Mech Dev. 1999 Jun;84(1-2):31-40
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5427985
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Gene symbol:
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Six3
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Gene name:
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sine oculis-related homeobox 3
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Probe:
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Six3 cDNA5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2E:
embryonic day 9.5
(more )
Specimen
2E:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2G:
embryonic day 9.5
(more )
Specimen
2G:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: optic stalk |
Present |
Widespread |
2G
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Expression was detected in the entire optic vesicle and optic stalk.
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TS15: optic vesicle |
Present |
Widespread |
2G
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Expression was detected in the entire optic vesicle and optic stalk.
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TS15: optic cup |
Present |
Regionally restricted |
2G
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Expression was detected in the entire retinal plate.
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TS15: optic cup inner layer |
Present |
Regionally restricted |
2G
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Expression was detected in the presumptive ventral neural retina.
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Specimen
3C:
embryonic day 9.5
(more )
Specimen
3C:
(close )
Genetic Background:
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NMRI
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Age:
|
embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS15: optic cup inner layer |
Present |
Regionally restricted |
3C
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Expression was detected in the presumptive neural retina.
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Specimen
3D:
embryonic day 9.5
(more )
Specimen
3D:
(close )
Genetic Background:
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NMRI
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: optic stalk |
Present |
Regionally restricted |
3D
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Expression was detected in the presumptive ventral optic stalk.
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TS15: diencephalon |
Present |
Regionally restricted |
3D
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Expression was detected in the pituitary/hypothalamic axis.
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Specimen
4B:
embryonic day 11.5
(more )
Specimen
4B:
(close )
Genetic Background:
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NMRI
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Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
4D:
embryonic day 11.5
(more )
Specimen
4D:
(close )
Genetic Background:
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NMRI
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
4F:
embryonic day 11.5
(more )
Specimen
4F:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4H:
embryonic day 11.5
(more )
Specimen
4H:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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