Gene Expression Data
Assay Details
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Assay
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Reference:
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J:56581
Daubas P, et al., Myf5 is a novel early axonal marker in the mouse brain and is subjected to post-transcriptional regulation in neurons. Development. 2000 Jan;127(2):319-31
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5433581
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
1B:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more  )
Specimen
1B:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
1B
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Expression is present in the rostral mesencephalon - ventral prosomere 1 region.
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Specimen
1D, E:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: This specimen was labeled with DiI post fixation. LacZ staining indicated by white arrows.
Specimen
1D, E:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This specimen was labeled with DiI post fixation. LacZ staining indicated by white arrows.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
1D
,
1E
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Expression is present in the rostral mesencephalon - ventral prosomere 1 region.
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Specimen
3A, B:
postnatal month 2; Myf5tm1Pas/Myf5 +
(more )
Specimen
3A, B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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postnatal month 2
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3C:
postnatal month 2; Myf5tm1Pas/Myf5 +
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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postnatal month 2
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Not shown:
embryonic day 14.5; Myf5tm1Pas/Myf5 +
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
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Expression is present in ventral regions of the brain, particularly at the level of the hypothalamus containing the prospective mammillary bodies.
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Specimen
Not shown:
embryonic day 18.5; Myf5tm1Pas/Myf5 +
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: diencephalon |
Present |
Regionally restricted |
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Expression is present in ventral regions of the brain, particularly at the level of the hypothalamus containing the prospective mammillary bodies.
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Specimen
7-Ngn2:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: salmon - Myf5; blue - Neurog2 (assay MGI:1352724). The section was cut through the ventral mesencephalon.
Specimen
7-Ngn2:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: salmon - Myf5; blue - Neurog2 (assay MGI:1352724). The section was cut through the ventral mesencephalon.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain mantle layer |
Present |
Regionally restricted |
7_Ngn2 right
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7_Ngn2 left
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Expression is present in neurons located on both sides of the ventral midline, exclusively in the outer portion of the mantle of the neuroepithelium.
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Specimen
7-NeuroM:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: salmon - Myf5; blue - Neurod4 (assay MGI:1352725). The section was cut through the ventral mesencephalon.
Specimen
7-NeuroM:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: salmon - Myf5; blue - Neurod4 (assay MGI:1352725). The section was cut through the ventral mesencephalon.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain mantle layer |
Present |
Regionally restricted |
7_NeuroM left
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7_NeuroM right
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Expression is present in neurons located on both sides of the ventral midline, exclusively in the outer portion of the mantle of the neuroepithelium.
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Specimen
7-NeuroD:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: salmon - Myf5; blue - Neurod1 (assay MGI:1352726). The section was cut through the ventral mesencephalon.
Specimen
7-NeuroD:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: salmon - Myf5; blue - Neurod1 (assay MGI:1352726). The section was cut through the ventral mesencephalon.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain mantle layer |
Present |
Regionally restricted |
7_NeuroD left
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7_NeuroD right
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Expression is present in neurons located on both sides of the ventral midline, exclusively in the outer portion of the mantle of the neuroepithelium.
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Specimen
7-Otlx2:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: salmon - Myf5; blue - Pitx2 (assay MGI:1352727). The section was cut through the ventral mesencephalon.
Specimen
7-Otlx2:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: salmon - Myf5; blue - Pitx2 (assay MGI:1352727). The section was cut through the ventral mesencephalon.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain mantle layer |
Present |
Regionally restricted |
7_Otlx2 right
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7_Otlx2 left
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Expression is present in neurons located on both sides of the ventral midline, exclusively in the outer portion of the mantle of the neuroepithelium.
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Specimen
Not shown:
embryonic day 12.5; Myf5tm1Pas/Myf5 +
(more )
Note: The section was cut through the ventral mesencephalon.
Specimen
Not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The section was cut through the ventral mesencephalon.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
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Expression is detected in the prosomeres 1 and 4.
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| TS20: midbrain |
Present |
Regionally restricted |
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Expression is detected in the prosomeres 1 and 4.
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Specimen
8A:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: salmon - Myf5; blue - Wnt1 (assay MGI:1352728).
Specimen
8A:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: salmon - Myf5; blue - Wnt1 (assay MGI:1352728).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: somite |
Present |
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8A
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| TS17: brain |
Present |
Regionally restricted |
8A
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Expression is present in subdomains.
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| TS17: midbrain |
Present |
Regionally restricted |
8A
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Expression is present in the ventral mesencephalon.
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Specimen
8C:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: salmon - Myf5; blue - Wnt1 (assay MGI:1352728).
Specimen
8C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: salmon - Myf5; blue - Wnt1 (assay MGI:1352728).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
8C
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Expression is present in the ventral mesencephalon.
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Specimen
8D:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: salmon - Myf5; blue - Shh (assay MGI:1352729).
Specimen
8D:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: salmon - Myf5; blue - Shh (assay MGI:1352729).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: somite |
Present |
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8D
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| TS17: brain |
Present |
Regionally restricted |
8D
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Expression is present in subdomains.
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| TS17: midbrain |
Present |
Regionally restricted |
8D
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Expression is present in the ventral mesencephalon.
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Specimen
8F:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: salmon - Myf5; blue - Shh (assay MGI:1352729).
Specimen
8F:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: salmon - Myf5; blue - Shh (assay MGI:1352729).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
8F
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Expression is present in the ventral mesencephalon.
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Specimen
10A:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: salmon - Myf5; blue - Sim1 (assay MGI:1352730).
Specimen
10A:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: salmon - Myf5; blue - Sim1 (assay MGI:1352730).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: somite |
Present |
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10A
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| TS17: brain |
Present |
Regionally restricted |
10A
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Expression is present in subdomains.
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| TS17: midbrain |
Present |
Regionally restricted |
10A
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Expression is present in the ventral mesencephalon.
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Specimen
10C:
embryonic day 10.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: blue - Myf5; silver grains - Sim1 (assay MGI:5433618).
Specimen
10C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Myf5; silver grains - Sim1 (assay MGI:5433618).
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