Probe:	Notch2 cDNA8
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS10: embryo	Strong	Regionally restricted	1B	Expression is in the notochord with an anterior boundary at the anterior margin of the node.
TS10: embryo mesoderm	Strong		1B

Structure	Level	Pattern	Image	Note
TS12: somite	Present	Regionally restricted	1F	Expression is in newly-formed somites.
TS12: notochord	Strong		1F
TS12: lateral plate mesoderm	Present		1F
TS12: neural fold	Present	Regionally restricted	1F	Expression is in the ventral-most cells.

Structure	Level	Pattern	Image	Note
TS12: node	Present		1H

Structure	Level	Pattern	Image	Note
TS11: notochordal plate	Strong		2_middle
TS11: neural ectoderm	Strong	Regionally restricted	2_middle	Expression is in the ventral-most cells of the neural groove.
TS11: embryo mesoderm	Strong	Regionally restricted	2_middle	Expression is in splanchnic mesoderm.

Structure	Level	Pattern	Image	Note
TS14: somite	Strong	Regionally restricted	3C	Expression is in the newly-formed somite and is rapidly downregulated beginning from the caudal region of the somite. Expression is in the dorsal epithelium.

Structure	Level	Pattern	Image	Note
TS14: somite	Present	Regionally restricted	3D	Expression is in the dorsal somite epithelium.

Structure	Level	Pattern	Image	Note
TS15: caudal neuropore	Strong	Regionally restricted	4B	Expression is in the dorsal lip of the anterior neuropore.
TS15: neural tube	Weak		4B
TS15: 1st branchial arch	Very strong	Regionally restricted	4B	Expression is in the anterior region.
TS15: surface ectoderm	Very strong	Regionally restricted	4B	Expression is in the surface epithelium overlying the telencephalon in the region where the olfactory pit will develop.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present		5B
TS17: infundibular recess of 3rd ventricle	Present		5B
TS17: neural tube roof plate	Present		5B
TS17: somite	Weak		5B
TS17: hindbrain	Present	Regionally restricted	5B	Expression is dorsal.
TS17: dorsal root ganglion	Absent		5B

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present	Regionally restricted	5E	Expression is in the lens epithelium.
TS17: optic cup	Present	Regionally restricted	5E	Expression is in epithelial cells at the junction between the pigmented layer and the neural retina.