Gene Expression Data
Assay Details
Assay
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Reference:
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J:65646
Tomotsune D, et al., Regulation of hoxb3 expression in the hindbrain and pharyngeal arches by rae28, a member of the mammalian polycomb group of genes. Mech Dev. 2000 Nov;98(1-2):165-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5435228
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Gene symbol:
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Hoxb3
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Gene name:
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homeobox B3
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Probe:
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Hox-2.7 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 8.0
(more )
Specimen
2A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS12: rhombomere |
Present |
Regionally restricted |
2A
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The anterior boundary of expression corresponds to the rhombomere 4/5 boundary.
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Specimen
2E:
(close )
Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS12: rhombomere |
Present |
Regionally restricted |
2E
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The anterior boundary of expression corresponds to the rhombomere 4/5 boundary as in wild type.
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Specimen
2B:
embryonic day 9.5
(more )
Specimen
2B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS15: rhombomere 4 |
Absent |
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2B
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The anterior boundary of expression corresponds to the rhombomere 4/5 boundary.
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TS15: rhombomere 5 |
Present |
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2B
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The anterior boundary of expression corresponds to the rhombomere 4/5 boundary.
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Specimen
2C:
(close )
Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: rhombomere 4 |
Absent |
|
2C
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The anterior boundary of expression corresponds to the rhombomere 4/5 boundary as in wild type.
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TS15: rhombomere 5 |
Present |
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2C
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The anterior boundary of expression corresponds to the rhombomere 4/5 boundary as in wild type.
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Specimen
2F:
embryonic day 9.5
(more )
Specimen
2F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
|
Not Specified
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Type:
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section
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Specimen
2G:
(close )
Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
3A:
embryonic day 11.5
(more )
Specimen
3A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
3B:
(close )
Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS19: hindbrain |
Present |
Regionally restricted |
3B
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The anterior boundary of ectopic expression extended to rhombomere 3 in the mutant embryo.
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS17: embryo |
Present |
|
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The expression pattern was affected in the mutant.
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TS17: rhombomere 4 |
Present |
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Expression was ectopic in rhombomere 4 in the mutant.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
not shown:
(close )
Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS20: embryo |
Present |
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The expression pattern was affected in the mutant.
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TS20: pons |
Present |
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The anterior boundary of ectopic expression had extended to the pons by E12.5.
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Specimen
5C:
embryonic day 9.5
(more )
Specimen
5C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
5D:
(close )
Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Phc1tm1Os/Phc1tm1Os
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS15: migrating neural crest |
Present |
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5D
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Expression was ectopic in regions with migrating neural crest cells from rhombomere 4.
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