Probe:	CrabpI probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	After whole-mount staining, embryos were post-fixed in 4% paraformaldehyde/0.2% glutaraldehyde before sectioning.

Structure	Level	Pattern	Image	Note
TS15: branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	2A	Expression was detected in migrating neural crest cells populating the branchial arches caudal to the first.
TS15: migrating neural crest	Present	Regionally restricted	2A	Expression was detected in migrating neural crest cells populating the branchial arches caudal to the first.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	2B	Expression in the migrating neural crest indicated an additional stream of cells that migrate into the first arch from rhombomere 4 in the mutant.
TS15: migrating neural crest	Present	Regionally restricted	2B	Expression in the migrating neural crest indicated an additional stream of cells that migrate into the first arch from rhombomere 4 in the mutant.

Structure	Level	Pattern	Image	Note
TS15: branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	2G	Expression was detected in neural crest cells in the second and more caudal arches.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	2H	Expression was detected in neural crest cells in the first arch from rhombomere 4. The labeled neural crest cells are adjacent to the mesodermal core and not to the vessels.