Gene Expression Data
Assay Details
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Assay
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Reference:
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J:96463
Moraes F, et al., Tbx1 is required for proper neural crest migration and to stabilize spatial patterns during middle and inner ear development. Mech Dev. 2005 Feb;122(2):199-212
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5435403
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Gene symbol:
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Sox10
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Gene name:
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SRY (sex determining region Y)-box 10
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Probe:
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Sox10 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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After whole-mount staining, embryos were post-fixed in 4% paraformaldehyde/0.2% glutaraldehyde before sectioning.
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Results
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Specimen
2C:
embryonic day 9.5
(more  )
Specimen
2C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: mesenchyme derived from neural crest |
Present |
Regionally restricted |
2C
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Expression was detected in neural crest cells contributing to cranial nerves and ganglia.
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| TS15: neural crest |
Present |
Regionally restricted |
2C
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Expression was detected in neural crest cells contributing to cranial nerves and ganglia.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tbx1tm1Pa/Tbx1tm1Pa
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
4C:
embryonic day 9.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst |
Present |
Regionally restricted |
4C
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Expression was detected in the lateral wall of the otocyst.
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Specimen
4D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tbx1tm1Pa/Tbx1tm1Pa
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst |
Present |
Regionally restricted |
4D
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Expression was detected in the lateral wall of the otocyst as in wild type.
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Specimen
5E:
embryonic day 10.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5E
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Expression was detected in the lateral wall of the otic vesicle and is excluded from antero-ventral areas.
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tbx1tm1Pa/Tbx1tm1Pa
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5F
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In the mutant expression was extended to more anterior areas of the otocyst lateral wall.
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Analysis Tools