Gene Expression Data
Assay Details
Assay
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Reference:
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J:42036
Tribioli C, et al., Bapx1: an evolutionary conserved homologue of the Drosophila bagpipe homeobox gene is expressed in splanchnic mesoderm and the embryonic skeleton. Mech Dev. 1997 Jul;65(1-2):145-62
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5438320
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Gene symbol:
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Nkx3-2
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Gene name:
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NK3 homeobox 2
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Probe:
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Bapx1 probe1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
8A/B:
embryonic day 8.0
(more )
Specimen
8A/B:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS12: somite |
Present |
Regionally restricted |
8A/B
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Expression was detected in the presclerotome region of the somite.
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TS12: lateral plate mesoderm |
Present |
Regionally restricted |
8A/B
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Expression was detected in lateral plate mesoderm overlying the future gut endoderm.
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Specimen
8C/D:
embryonic day 8.5
(more )
Specimen
8C/D:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS13: lateral plate mesoderm |
Strong |
Regionally restricted |
8C/D
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Expression was detected in lateral plate mesoderm surrounding the midgut epithelium.
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TS13: somite |
Present |
Regionally restricted |
8C/D
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Expression was detected in the sclerotome.
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Specimen
8E/F:
embryonic day 9.0
(more )
Specimen
8E/F:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.0
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
8G/H:
embryonic day 9.0
(more )
Specimen
8G/H:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
8I/J:
embryonic day 9.5
(more )
Specimen
8I/J:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
8K/L:
embryonic day 9.5
(more )
Specimen
8K/L:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS15: 1st branchial arch |
Present |
Regionally restricted |
8K/L
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Highest intensity was in the inferior portion on the first branchial arch.
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Specimen
9A/B:
embryonic day 10.5
(more )
Specimen
9A/B:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
9C/D:
embryonic day 10.5
(more )
Specimen
9C/D:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
9E/F:
embryonic day 10.5
(more )
Specimen
9E/F:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Specimen
9G/H:
embryonic day 10.5
(more )
Specimen
9G/H:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Specimen
9I/J:
embryonic day 10.5
(more )
Specimen
9I/J:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: sclerotome |
Strong |
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9I/J
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TS17: hindgut mesentery |
Strong |
Regionally restricted |
9I/J
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Expression was detected in the lesser omentum (ventral mesoderm of the stomach) and in discreet patches surrounding the hindgut.
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TS17: ventral mesogastrium |
Strong |
Regionally restricted |
9I/J
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Expression was detected in the lesser omentum (ventral mesoderm of the stomach) and in discreet patches surrounding the hindgut.
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Specimen
9K/L:
embryonic day 12.5
(more )
Specimen
9K/L:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
10A/B:
embryonic day 12.5
(more )
Specimen
10A/B:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindgut mesentery |
Present |
Regionally restricted |
10A/B
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Expression was detected in the lesser omentum (ventral mesoderm of the stomach) and in discreet patches surrounding the hindgut.
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TS20: ventral mesogastrium |
Present |
Regionally restricted |
10A/B
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Expression was detected in the lesser omentum (ventral mesoderm of the stomach) and in discreet patches surrounding the hindgut.
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TS20: axial skeleton |
Strong |
Regionally restricted |
10A/B
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Strong expression was detected in the prevertebrae. Expression was detected in both the vertebral body and the intervertebral disc.
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TS20: hindlimb pre-cartilage condensation |
Strong |
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10A/B
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Specimen
10C/D:
embryonic day 12.5
(more )
Specimen
10C/D:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
10E/F:
embryonic day 14.5
(more )
Specimen
10E/F:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
10G/H:
embryonic day 14.5
(more )
Specimen
10G/H:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
10I/J:
embryonic day 16.5
(more )
Note: This pane appears to be mislabeled in the figure legend.
Specimen
10I/J:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
|
Note:
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This pane appears to be mislabeled in the figure legend.
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: embryo |
Present |
Regionally restricted |
10I/J
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Expression was detected qualitatively in all the same structures as earlier stages, but by E16.5 there is a significant decrease in the level of expression.
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TS24: vertebra |
Present |
Regionally restricted |
10I/J
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Expression was strongest in the most caudal prevertebrae and has decreased significantly in more rostral prevertebrae.
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Specimen
10K/L:
embryonic day 16.5
(more )
Note: This pane appears to be mislabeled in the figure legend.
Specimen
10K/L:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
This pane appears to be mislabeled in the figure legend.
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Structure |
Level |
Pattern |
Image |
Note |
TS24: limb skeleton |
Present |
Regionally restricted |
10K/L
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Expression was strongest in the most distal cartilaginous condensations, and decreases in a distoproximal gradient.
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TS24: limb digit |
Strong |
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10K/L
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Specimen
11A/B:
embryonic day 10.0
(more )
Specimen
11A/B:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
11E/F:
embryonic day 12.5
(more )
Specimen
11E/F:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: tail mesenchyme |
Present |
Regionally restricted |
11E/F
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Expression was detected in the mesoderm surrounding the notochord. Expression was highest in cells closest to the notochord and decreased with increasing distance from the notochord.
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Specimen
11J/K:
embryonic day 16.5
(more )
Specimen
11J/K:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
12A/B:
embryonic day 12.5
(more )
Specimen
12A/B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: axial skeleton |
Present |
Regionally restricted |
12A/B
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Expression was detected in the prevertebrae of the entire axial skeleton.
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TS20: somite |
Present |
Regionally restricted |
12A/B
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Expression was more densely localized in the caudal half of the somite.
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Specimen
12F/G:
embryonic day 12.5
(more )
Specimen
12F/G:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
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