Probe:	Hes3 cDNA6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Regionally restricted	1A	Expressed in the embryonic ectoderm, just ahead of the node and more caudally flanking the primitive streak.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	1B	The anterior boundary was in the region of the prospective hindbrain. Expressed in the neural plate ahead of the node and in ectoderm lateral to the primitive streak.
TS11: future spinal cord neural plate	Present	Regionally restricted	1B	Expressed in the neural plate ahead of the node.
TS11: future brain	Present	Regionally restricted	1B	The anterior boundary was in the region of the prospective hindbrain.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Strong	Regionally restricted	1E	Expressed in the region of the dorsal mesencephalic/metencephalic boundary.
TS14: midbrain-hindbrain junction	Strong	Regionally restricted	1E	Expressed in the region of the dorsal mesencephalic/metencephalic boundary.
TS14: future hindbrain	Strong	Regionally restricted	1E	Expressed in the region of the dorsal mesencephalic/metencephalic boundary.
TS14: future spinal cord neural plate	Strong		1E
TS14: rhombomere 4	Strong		1E
TS14: rhombomere 6	Strong		1E
TS14: rhombomere 7	Strong		1E
TS14: embryo ectoderm	Present	Regionally restricted	1E	Expressed in ectoderm adjacent to the primitive streak.
TS14: neural tube floor plate	Absent		1E
TS14: rhombomere 5	Absent		1E

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Strong	Regionally restricted	1F	Expressed in the region of the dorsal mesencephalic/metencephalic boundary.
TS14: future hindbrain	Strong	Regionally restricted	1F	Expressed in the region of the dorsal mesencephalic/metencephalic boundary.
TS14: midbrain-hindbrain junction	Strong	Regionally restricted	1F	Expressed in the region of the dorsal mesencephalic/metencephalic boundary.
TS14: future spinal cord neural plate	Strong		1F
TS14: rhombomere 4	Strong		1F
TS14: rhombomere 6	Strong		1F
TS14: rhombomere 7	Strong		1F
TS14: embryo ectoderm	Present	Regionally restricted	1F	Expressed in ectoderm adjacent to the primitive streak.
TS14: rhombomere 5	Absent		1F

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	2A	Embryos display a gap (rhombomere 03) in hindbrain expression.
TS13: rhombomere 3	Absent		2A

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	2B	Embryos display a gap (rhombomere 03, positive for Krox20) in hindbrain expression.
TS13: rhombomere 3	Absent		2B

Structure	Level	Pattern	Image	Note
TS14: rhombomere 1	Present		2D	Expression is strongest among all rhombomeres.
TS14: rhombomere 2	Weak		2D
TS14: rhombomere 4	Weak		2D
TS14: rhombomere 6	Weak		2D
TS14: rhombomere 7	Weak		2D
TS14: rhombomere 3	Absent		2D
TS14: rhombomere 5	Absent		2D
TS14: embryo ectoderm	Present	Regionally restricted	2D	Expressed in ectoderm adjacent to the primitive streak.
TS14: future spinal cord neural plate	Present		2D

Structure	Level	Pattern	Image	Note
TS14: rhombomere 1	Present		2E	Expression is strongest among all rhombomeres.
TS14: rhombomere 2	Weak		2E
TS14: rhombomere 4	Weak		2E
TS14: rhombomere 6	Weak		2E
TS14: rhombomere 7	Weak		2E
TS14: rhombomere 3	Absent		2E
TS14: rhombomere 5	Absent		2E

Structure	Level	Pattern	Image	Note
TS14: rhombomere 1	Present		2G	Expression is strongest among all rhombomeres.
TS14: rhombomere 2	Weak		2G
TS14: rhombomere 4	Weak		2G
TS14: rhombomere 6	Weak		2G
TS14: rhombomere 7	Weak		2G
TS14: rhombomere 3	Absent		2G
TS14: rhombomere 5	Absent		2G
TS14: embryo ectoderm	Present	Regionally restricted	2G	Expressed in ectoderm adjacent to the primitive streak.
TS14: future spinal cord neural plate	Present		2G

Structure	Level	Pattern	Image	Note
TS14: rhombomere 1	Present		2H	Expression is strongest among all rhombomeres.
TS14: rhombomere 2	Weak		2H
TS14: rhombomere 4	Weak		2H
TS14: rhombomere 6	Weak		2H
TS14: rhombomere 7	Weak		2H
TS14: rhombomere 3	Absent		2H
TS14: rhombomere 5	Absent		2H

Structure	Level	Pattern	Image	Note
TS14: rhombomere 1	Present		2J	Expression is strongest among all rhombomeres.
TS14: rhombomere 2	Weak		2J
TS14: rhombomere 4	Weak		2J
TS14: rhombomere 6	Weak		2J
TS14: rhombomere 7	Weak		2J
TS14: rhombomere 3	Absent		2J
TS14: rhombomere 5	Absent		2J

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	3A	Expressed in the dorsal region of the mesencephalic/metencephalic boundary.
TS14: future midbrain	Present	Regionally restricted	3A	Expressed in the dorsal region of the mesencephalic/metencephalic boundary.
TS14: midbrain-hindbrain junction	Present	Regionally restricted	3A	Expressed in the dorsal region of the mesencephalic/metencephalic boundary.
TS14: embryo ectoderm	Strong	Regionally restricted	3A	Expressed in ectoderm adjacent to the primitive streak.
TS14: future spinal cord neural plate	Strong		3A
TS14: rhombomere 4	Weak		3A	The expression is quite low.
TS14: rhombomere 6	Weak		3A	The expression is quite low.
TS14: rhombomere 7	Weak		3A	The expression is quite low.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Weak		4A
TS15: future hindbrain	Weak	Regionally restricted	4A	Expressed in the rostral part of the metencephalon.
TS15: tail neural tube	Weak	Regionally restricted	4A	Expressed in the dorsal part of the open neural tube in the tail region.
TS15: midbrain-hindbrain junction	Weak	Regionally restricted	4A	Expressed in the dorsal part of the midhindbrain boundary.

Structure	Level	Pattern	Image	Note
TS16: future midbrain	Present	Scattered	4B	Expressed more rostrally in the mesencephalon.
TS16: future hindbrain	Present		4B	Strongest expression is in the cerebello-mesencephalic flexure.
TS16: midbrain-hindbrain junction	Present		4B	Strongest expression is in the cerebello-mesencephalic flexure.
TS16: neural tube	Present	Graded	4B	Expressed in a rostral to caudal gradient.

Structure	Level	Pattern	Image	Note
TS16: future forebrain	Present		4C	Stronger expression throughout the brain.
TS16: future hindbrain	Present		4C	Stronger expression throughout the brain.
TS16: future midbrain	Present		4C	Stronger expression throughout the brain.
TS16: midbrain-hindbrain junction	Present		4C	Stronger expression throughout the brain.
TS16: neural tube	Present		4C	Stronger expression throughout the brain.

Structure	Level	Pattern	Image	Note
TS16: future hindbrain	Present	Regionally restricted	4G	Expressed in the dorsal-lateral part of the metencephalon.
TS16: neural tube	Present	Regionally restricted	4G	Expressed in the dorsal-lateral part.

Structure	Level	Pattern	Image	Note
TS16: future hindbrain	Present	Regionally restricted	4H	Expressed in the dorsal-lateral part of the myelencephalon.
TS16: neural tube	Present	Regionally restricted	4H	Expressed in the dorsal-lateral part.

Structure	Level	Pattern	Image	Note
TS16: future midbrain	Present	Regionally restricted	4J	Expressed in two bands, one just rostral of the mesencephalic/metencephalic boundary and one slightly more anterior.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	4K	The strongest signal occurs rostral to the isthmus, in the inferior colliculus.

Structure	Level	Pattern	Image	Note
TS20: nasal cavity respiratory epithelium	Present		5A	Expressed in the nasosensory epithelia.
TS20: olfactory epithelium	Present		5A	Expressed in cells lining the olfactory pit.
TS20: telencephalon ventricular layer	Present	Regionally restricted	5A	Expressed in the dorsal ventricular zone, the roof of the telencephalon.
TS20: midbrain roof plate	Present	Regionally restricted	5A	Expressed in the caudal region.
TS20: cranial nerve	Present		5A
TS20: diencephalon lateral wall	Present		5A
TS20: diencephalon roof plate	Present		5A
TS20: lens vesicle epithelium	Present		5A
TS20: midbrain-hindbrain junction	Present		5A
TS20: pigmented retinal epithelium	Present		5A
TS20: diencephalon floor plate	Weak		5A
TS20: trigeminal nerve	Present	Regionally restricted	5A	Expressed in semilunar ganglia of the trigeminal nerve.
TS20: facial ganglion	Present		5A	Expressed in accousticofacialis ganglia VII.
TS20: vestibulo-cochlear ganglion	Present		5A	Expressed in accousticofacialis ganglia VIII.
TS20: inner ear epithelium	Present	Single cells	5A	Expressed in a few cells lining the otic vesicle.
TS20: medulla oblongata ventricular layer	Present	Regionally restricted	5A	Expressed in the ventricular zone above the sulcus limitans in the myelencephalon.
TS20: medulla oblongata floor plate	Present		5A	Expressed in the floor plate in the myelencephalon.
TS20: epidermis	Present	Regionally restricted	5A	Expressed in the basal layer of the skin.
TS20: midbrain ventricular layer	Present	Regionally restricted	5A	Strongest expression was in the lateral walls, just above the sulcus limitans.
TS20: hindbrain floor plate	Strong	Single cells	5A	Expressed in a few cells.
TS20: spinal cord floor plate	Strong	Single cells	5A	Expressed in a few cells.
TS20: hindbrain ventricular layer	Present	Regionally restricted	5A	Expressed in the caudal part.
TS20: spinal cord ventricular layer	Present	Regionally restricted	5A	Expressed in the caudal part.

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Present	Regionally restricted	5B	Strongest expression is in the dorsal-most part of the spinal cord, in cells of the ventricular zone lining the lumen.
TS21: spinal cord floor plate	Present	Single cells	5B	Expressed in a few cells.
TS21: dorsal root ganglion	Present		5B

Structure	Level	Pattern	Image	Note
TS22: vibrissa follicle	Present		5D	Expressed in the whisker follicles.
TS22: nasal cavity epithelium	Present		5D	Expressed in the nasosensory epithelia.
TS22: tooth	Present		5D	Expressed in the toothbud progenitor.
TS22: brainstem	Present		5D
TS22: cerebral cortex ventricular layer	Present		5D
TS22: hair follicle	Present		5D
TS22: inferior colliculus	Present		5D
TS22: trigeminal ganglion	Present		5D
TS22: inner ear epithelium	Present		5D	Expressed in the neuroepithelium of the otic vesicle.
TS22: cochlear duct epithelium	Present		5D	Expressed in cells lining the cochlea.
TS22: midbrain tegmentum	Present	Regionally restricted	5D	Expressed in the post-tegmental neuroepithelium.
TS22: cerebellum	Present	Regionally restricted	5D	Expressed in the external granular layer of the prospective cerebellum.
TS22: tectum	Present	Regionally restricted	5D	Expressed in the tectal neuroepithelium.

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis pars intermedia	Present		5E	Expressed in the pars intermedia of the developing pituitary.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present	Regionally restricted	5F	Expressed in the external granular layer of the prospective cerebellum.
TS22: medulla oblongata	Present	Regionally restricted	5F	Expressed in the dorsal medulla.
TS22: 4th ventricle choroid plexus	Present		5F

Structure	Level	Pattern	Image	Note
TS22: adrenal medulla	Present		5G
TS22: gut epithelium	Present		5G
TS22: liver	Present		5G
TS22: lung epithelium	Present		5G
TS22: metanephros	Present		5G
TS22: renal cortex	Present		5G
TS22: dorsal root ganglion	Present		5G

Structure	Level	Pattern	Image	Note
TS24: vibrissa follicle	Present		6A	Expressed in the whisker follicles.
TS24: tooth epithelium	Present		6A	Expressed in the epithelia of the toothbud progenitor.
TS24: cerebral cortex ventricular layer	Present		6A
TS24: cochlear duct epithelium	Present		6A
TS24: hair follicle	Present		6A
TS24: nasal cavity epithelium	Present		6A
TS24: cerebellum	Present	Regionally restricted	6A	Expressed in the external granular layer of the cerebellum.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present		6B	Expressed in the basal layer of the skin.
TS24: hair follicle	Present		6B
TS24: vibrissa follicle	Present	Regionally restricted	6B	Expressed in the inner root sheath of the whisker follicles.

Structure	Level	Pattern	Image	Note
TS24: inferior colliculus	Present	Regionally restricted	6C	Expressed in the ventricular zone of the inferior colliculus.
TS24: cerebellum	Present	Regionally restricted	6C	Expressed in the external granular layer of the cerebellum.
TS24: tectum	Present	Regionally restricted	6C	Expressed in the tectal neuroepithelium.
TS24: choroid plexus	Present		6C

Structure	Level	Pattern	Image	Note
TS16: tail neural plate	Present

Structure	Level	Pattern	Image	Note
TS20: midbrain ventricular layer	Present	Regionally restricted		Expressed in the lateral walls, just above the sulcus limitans, in the ventricular zone next to the lumen.
TS20: spinal cord marginal layer	Present	Regionally restricted		Expressed in the ventral horn.

Structure	Level	Pattern	Image	Note
TS22: salivary gland	Present
TS22: spinal cord ventricular layer	Present
TS22: thymus primordium	Present	Regionally restricted		Expressed in the thymic epithelia.

Structure	Level	Pattern	Image	Note
TS24: thymus	Present	Regionally restricted		Expressed in the thymic epithelia.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present