Gene Expression Data
Assay Details
Assay
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Reference:
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J:39657
Lobe CG, Expression of the helix-loop-helix factor, Hes3, during embryo development suggests a role in early midbrain-hindbrain patterning. Mech Dev. 1997 Mar;62(2):227-37
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5444151
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Gene symbol:
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Ascl1
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Gene name:
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achaete-scute family bHLH transcription factor 1
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Probe:
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Mash1 cDNA9
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4D:
embryonic day 9.0
(more )
Specimen
4D:
(close )
Genetic Background:
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FVB/N
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: future midbrain |
Present |
Regionally restricted |
4D
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Expressed in the dorsal part of the mesencephalon.
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Specimen
4E:
embryonic day 9.5
(more )
Specimen
4E:
(close )
Genetic Background:
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FVB/N
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4F:
embryonic day 9.5
(more )
Note: A slightly older embryo than that shown in 4B.
Specimen
4F:
(close )
Genetic Background:
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FVB/N
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
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A slightly older embryo than that shown in 4B.
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Structure |
Level |
Pattern |
Image |
Note |
TS15: future forebrain |
Present |
|
4F
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Expression is stronger throughout the central nervous system, similar to Hes3 expression at the same stage
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TS15: future hindbrain |
Present |
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4F
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Expression is stronger throughout the central nervous system, similar to Hes3 expression at the same stage
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TS15: future midbrain |
Present |
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4F
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Expression is stronger throughout the central nervous system, similar to Hes3 expression at the same stage
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TS15: neural tube |
Present |
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4F
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Expression is stronger throughout the central nervous system, similar to Hes3 expression at the same stage
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Specimen
4I:
embryonic day 9.5
(more )
Note: Cross-sections of the embryo shown in (F).
Specimen
4I:
(close )
Genetic Background:
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FVB/N
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Cross-sections of the embryo shown in (F).
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Specimen
5C:
embryonic day 12.5
(more )
Specimen
5C:
(close )
Genetic Background:
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either: CD-1 or FVB/N
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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