Probe:	IMAGE clone 552716
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: node	Present		3A
TS13: notochord	Present		3A

Structure	Level	Pattern	Image	Note
TS13: node	Present		3A inset
TS13: notochord	Present		3A inset

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present	Regionally restricted	3B	Expression is present rostrally.
TS15: neural tube lateral wall	Present	Regionally restricted	3B	Expression is present in the rostral region that will form motor neurons.
TS15: future hindbrain roof plate	Present		3B
TS15: neural tube roof plate	Present		3B
TS15: diencephalon floor plate	Present		3B	Expression is present in the ventral midline.
TS15: future midbrain floor plate	Present		3B	Expression is present in the ventral midline.
TS15: notochord	Present		3B

Structure	Level	Pattern	Image	Note
TS17: notochord	Present		3C
TS17: neural tube floor plate	Present		3C
TS17: neural tube roof plate	Present		3C
TS17: neural tube lateral wall	Present	Regionally restricted	3C	Expression is present in the region that will form motor neurons.
TS17: hindbrain	Present	Regionally restricted	3C	Expression is present in the dorsal edges of the myelencephalon and metencephalon.
TS17: hindbrain floor plate	Present		3C
TS17: midbrain floor plate	Present		3C
TS17: limb	Present	Regionally restricted	3C	Expression is present in the proximal anterior and posterior margins of the end of the limb bud.
TS17: trunk	Present	Regionally restricted	3C	Expression is present in flank tissue between the limbs.
TS17: otocyst	Present	Widespread	3C
TS17: lens pit	Present		3C

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present		3D
TS20: spinal cord roof plate	Present		3D
TS20: basal columns	Present	Regionally restricted	3D	Expression is present in the region that will form motor neurons.
TS20: medulla oblongata	Present	Regionally restricted	3D	Expression is present in the dorsal edges.
TS20: metencephalon	Present	Regionally restricted	3D	Expression is present in the dorsal edges.
TS20: trunk	Present	Regionally restricted	3D	Expression is present in a continuous band in flank tissue between the fore- and hindlimbs.
TS20: otic capsule	Present	Widespread	3D

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present		3E
TS21: hindbrain	Present		3E
TS21: midbrain	Present		3E
TS21: telencephalon	Absent		3E
TS21: spinal cord floor plate	Present		3E
TS21: spinal cord roof plate	Present		3E
TS21: spinal cord mantle layer	Present	Regionally restricted	3E	Expression is present in somatic and autonomic preganglionic motor neurons.
TS21: external auditory meatus	Present		3E
TS21: limb interdigital region	Strong		3E	As the digits separate, expression ceases first in the central midline of each interdigital region and becomes restricted to the lateral margins of each digit.
TS21: limb digit	Present	Regionally restricted	3E	Expression is present in the metacarpophalangeal joints.

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Present		4A
TS19: hindbrain floor plate	Strong		4A	Expression is observed in the floor plate up to the rostral end of the midbrain.
TS19: midbrain floor plate	Strong		4A	Expression is observed in the floor plate up to the rostral end of the midbrain.
TS19: diencephalon	Present	Regionally restricted	4A	Expression is present in a patch rostral to the optic stalk.
TS19: diencephalon floor plate	Present		4A
TS19: diencephalon roof plate	Present		4A
TS19: hindbrain	Strong	Regionally restricted	4A	Expression is present in the rostal half.
TS19: midbrain	Strong	Regionally restricted	4A	Expression is present in the rostal half.
TS19: midbrain roof plate	Present		4A
TS19: telencephalon	Absent		4A

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Strong		4C
TS21: hindbrain	Strong	Regionally restricted	4C	Expression is present in the rostal half.
TS21: midbrain	Strong	Regionally restricted	4C	Expression is present in the rostal half.
TS21: diencephalon roof plate	Present		4C
TS21: hindbrain floor plate	Present		4C
TS21: midbrain floor plate	Present		4C
TS21: midbrain roof plate	Present		4C
TS21: diencephalon floor plate	Present		4C
TS21: diencephalon	Present	Regionally restricted	4C	Expression is present in a small region rostral to the optic stalk.
TS21: tongue	Present	Regionally restricted	4C	Expression is present in the ventral tongue.
TS21: telencephalon	Absent		4C

Structure	Level	Pattern	Image	Note
TS21: diencephalon floor plate	Strong		4D	Expression is observed in the roof plate and floor plate in the rostral midbrain and the floor of the diencephalon.
TS21: midbrain floor plate	Strong		4D	Expression is observed in the roof plate and floor plate in the rostral midbrain and the floor of the diencephalon.
TS21: midbrain roof plate	Strong		4D	Expression is observed in the roof plate and floor plate in the rostral midbrain and the floor of the diencephalon.

Structure	Level	Pattern	Image	Note
TS21: hypoglossal nerve	Present		4E	Expression is present in the motor nuclei.
TS21: hindbrain floor plate	Strong		4E	Expression is present in the floor plate toward the rostral end.
TS21: hindbrain	Strong	Regionally restricted	4E	Expression is present in two distinct cells groups on rhombomere 5; a streak of staining connects this staining to the midline.

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Strong		4F
TS19: neural tube roof plate	Strong		4F
TS19: notochord	Strong		4F
TS19: neural tube basal columns	Strong		4F	Expression is present in the motor neurons.

Structure	Level	Pattern	Image	Note
TS15: neural tube roof plate	Present		4H
TS15: notochord	Present		4H
TS15: neural tube floor plate	Absent		4H	There is no expression in the caudal neural tube.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		4I
TS17: neural tube roof plate	Present		4I
TS17: notochord	Present		4I
TS17: neural tube lateral wall	Present	Regionally restricted	4I	Expression is present in the motor neurons.

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present		4J
TS20: spinal cord roof plate	Present		4J
TS20: basal columns	Present	Regionally restricted	4J	Expression is present in the motor neurons and in pre-migratory motor neurons near the ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Present		4K
TS21: spinal cord roof plate	Present		4K
TS21: spinal cord mantle layer	Present		4K	Expression is present in somatic and autonomic preganglionic motor neurons.

Structure	Level	Pattern	Image	Note
TS24: spinal cord floor plate	Present		4L
TS24: spinal cord roof plate	Present		4L
TS24: basal columns	Present		4L	Expression is present in the motor neurons.

Structure	Level	Pattern	Image	Note
TS20: mesonephros	Weak	Regionally restricted	5A	Expression is present within the mesonephros apposing the gonadal component.
TS20: metanephros	Present		5A
TS20: gonad	Absent		5A

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		5B
TS20: mesenchyme of metanephros	Present		5B

Structure	Level	Pattern	Image	Note
TS20: mesenchyme of metanephros	Present	Regionally restricted	5C	Expression is restricted to the mesenchyme adjacent to the invading ureteric bud.

Structure	Level	Pattern	Image	Note
TS20: mesenchyme of metanephros	Present	Regionally restricted	5E	Expression is limited to the mesenchyme directly adjacent to the ureteric bud but not within the bud.
TS20: primitive collecting duct	Absent		5E	There is no expression in the ureteric bud.

Structure	Level	Pattern	Image	Note
TS22: genital tubercle	Present	Regionally restricted	5H	Expression is present in the lateral edges on either side of the base of the genital tubercle. This expression does not show sexual dimorphism. (The sex of this specimen was not reported.)

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Present	Regionally restricted	5I	Expression is present between the comma-shaped bodies and the collecting ducts derived from the ureteric bud.
TS21: gonad	Absent		5I
TS21: primitive collecting duct	Absent		5I

Structure	Level	Pattern	Image	Note
TS22: nephrogenic interstitium	Present		5K
TS22: adrenal gland	Present		5K
TS22: renal medulla	Absent		5K

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	6A	Expression is present in the proximal anterior and posterior margins of the end of the limb bud.

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region	Strong		6B
TS20: hand	Present	Regionally restricted	6B	Expression is present in the anterior and posterior necrotic zones prior to apoptosis.
TS20: forelimb digit mesenchyme	Present	Regionally restricted	6B	Expression is present in the metacarpophalangeal joints.

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region	Strong	Regionally restricted	6C	Expression is present contracting away from the midline, "painting" the edges of the digits.
TS21: hand	Present	Regionally restricted	6C	Expression is present in the anterior and posterior margins of the outer-most digits.
TS21: forelimb digit mesenchyme	Present	Regionally restricted	6C	Expression is present in the metacarpophalangeal joints.

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region mesenchyme	Present		6D

Structure	Level	Pattern	Image	Note
TS22: forelimb digit mesenchyme	Present	Regionally restricted	6H	Expression is present in the mesenchyme immediately below the surface ectoderm of the marginal digits.
TS22: forelimb digit skin	Absent		6H

Structure	Level	Pattern	Image	Note
TS14: notochord	Present	Widespread		Expression is present along the entire length.
TS14: optic vesicle	Weak
TS14: nose	Present			Expression is present in the nasal pit.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted		Expression is present in the anterior rim.

Structure	Level	Pattern	Image	Note
TS16: medial-nasal process	Present			Expression is present in the medial side of the nasal pit.

Structure	Level	Pattern	Image	Note
TS18: gonad primordium	Present	Diffuse		Expression is detected in the caudal end of the urogenital ridge.
TS18: gut	Present
TS18: stomach	Present

Structure	Level	Pattern	Image	Note
TS19: handplate mesenchyme	Weak	Regionally restricted		Expression is present just below the apical ectodermal ridge, extending into a single posterior interdigital domain.
TS19: stomach	Present	Regionally restricted		Expression is restricted to the lateral wall.
TS19: gut	Present

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Present	Regionally restricted		Expression is present on either side of the base of the genital tubercle. This expression does not show sexual dimorphism.
TS20: urinary system mesentery	Present			Expression is present in the mesentery containing the renal arteries.
TS20: gut mesentery	Present			Expression is in the mesentery between the intestinal loops.

Structure	Level	Pattern	Image	Note
TS21: eyelid	Present			Expression extends completely around the eye.

Structure	Level	Pattern	Image	Note
TS22: genital tubercle of female	Present			Expression is present on either side of the base of the genital tubercle. This expression does not show sexual dimorphism.

Structure	Level	Pattern	Image	Note
TS22: genital tubercle of male	Present			Expression is present on either side of the base of the genital tubercle. This expression does not show sexual dimorphism.

Structure	Level	Pattern	Image	Note
TS22: urinary system mesentery	Present			Expression is present in the mesentery containing the renal arteries.
TS22: hindlimb digit	Present	Regionally restricted		Residual staining is detectable on the lateral margins of the more posterior digits of the hindlimbs.
TS22: gut mesentery	Trace			Expression is present in the mesentery between intestinal loops.

Structure	Level	Pattern	Image	Note
TS26: nephrogenic interstitium	Present