Probe:	Ihh cDNA14
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS20: hand pre-cartilage condensation	Present	Regionally restricted	1C	Expression in a subset of col2-positive cells.

Structure	Level	Pattern	Image	Note
TS22: femur cartilage condensation	Present	Regionally restricted	1G	Expression in mature hypertrophic chondrocytes adjacent to ihh-expressing cells.

Structure	Level	Pattern	Image	Note
TS24: forelimb phalanx	Present	Regionally restricted	1L	Expression restricted to hypertrophic chondrocytes in developing digits.

Structure	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate hypertrophic zone	Present		1Q	Expression restricted to upper zone of hypertrophic chondrocytes in growth plate.
TS28: long bone epiphysis	Absent		1Q

Structure	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate	Absent
TS28: long bone epiphysis	Absent

Structure	Level	Pattern	Image	Note
TS22: vertebral cartilage condensation	Present	Regionally restricted	3B	Expression detected in cartilage primordia of vertebral bodies.

Structure	Level	Pattern	Image	Note
TS24: rib	Present	Regionally restricted	3E	Expression in mature chondrocytes surrounding center of vertebral bodies, and in ribs.
TS24: vertebra	Present	Regionally restricted	3E	Expression in mature chondrocytes surrounding center of vertebral bodies, and in ribs.

Structure	Level	Pattern	Image	Note
TS23: basioccipital bone	Present		3H
TS23: exoccipital bone	Present		3H

Structure	Level	Pattern	Image	Note
TS25: mandible	Present	Regionally restricted	3J	Expression in proximal mandible and in sclerotic ossicles derived from proximal region of Meckel's cartilage.

Structure	Level	Pattern	Image	Note
TS25: Meckel's cartilage	Absent		3L