Hoxd12 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:75699 Albrecht AN, et al., The synpolydactyly homolog (spdh) mutation in the mouse -- a defect in patterning and growth of limb cartilage elements. Mech Dev. 2002 Mar;112(1-2):53-67
Assay type:	RNA in situ
MGI Accession ID:	MGI:5446451
Gene symbol:	Hoxd12
Gene name:	homeobox D12

Probe:	Hoxd12 probe9
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen not shown 10.5 wt: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present

Specimen not shown 10.5 spdh/spdh: embryonic day 10.5; Hoxd13^spdh/Hoxd13^spdh (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted		Authors report normal expression domains with respect to anterior and posterior borders, but with reduced overall area of expression compared with wildtype.

Specimen 2 Hoxd12 11.5 wt: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: handplate	Present	Regionally restricted	2_Hoxd12 11.5 wt/wt

Specimen 2 Hoxd12 11.5 spdh/spdh: embryonic day 11.5; Hoxd13^spdh/Hoxd13^spdh (more

)

Structure	Level	Pattern	Image	Note
TS19: handplate	Present	Regionally restricted	2_Hoxd12 11.5 spdh/spdh	Reduced expression domain with normal anterior and posterior borders.

Specimen 2 Hoxd12 12.5 wt: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb digit 2 mesenchyme	Present	Regionally restricted	2_Hoxd12 12.5 wt/wt	Expression more restricted to perichondrial region of cartilaginous anlagen of digits 2-4 and presumptive joint spaces of those digits.
TS20: forelimb digit 3 mesenchyme	Present	Regionally restricted	2_Hoxd12 12.5 wt/wt	Expression more restricted to perichondrial region of cartilaginous anlagen of digits 2-4 and presumptive joint spaces of those digits.
TS20: forelimb digit 4 mesenchyme	Present	Regionally restricted	2_Hoxd12 12.5 wt/wt	Expression more restricted to perichondrial region of cartilaginous anlagen of digits 2-4 and presumptive joint spaces of those digits.

Specimen 2 Hoxd12 12.5 spdh/spdh: embryonic day 12.5; Hoxd13^spdh/Hoxd13^spdh (more

)

Structure	Level	Pattern	Image	Note
TS20: hand	Present	Regionally restricted	2_Hoxd12 12.5 spdh/spdh	Reduced expression domain with normal anterior and posterior borders. Expression pattern comparable to that seen at E11.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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