Probe:	Nkx3-1 probe2
Probe preparation:	labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: paraxial mesenchyme	Present	Regionally restricted	1A	Expression in paraxial mesoderm adjacent to neural fold.

Structure	Level	Pattern	Image	Note
TS15: sclerotome	Present		1F

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present	Regionally restricted	1H	Expression located adjacent to cells expressing Pecam1, suggesting expressing cells were not endothelial cells.

Structure	Level	Pattern	Image	Note
TS19: dorsal aorta	Present	Regionally restricted	1J	Expression in a subpopulation of vascular smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS19: common dorsal aorta	Present		2A	Expression in abdominal aorta and vertebral vessels.
TS19: vertebral artery	Present		2A	Expression in abdominal aorta and vertebral vessels.

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis	Present	Regionally restricted	2B	Expression in the distal epithelium of the tongue and Rathke's pouch (anterior pituitary).
TS20: tongue epithelium	Present	Regionally restricted	2B	Expression in the distal epithelium of the tongue and Rathke's pouch (anterior pituitary).

Structure	Level	Pattern	Image	Note
TS21: adenohypophysis	Present	Regionally restricted	2C	Expression in the distal epithelium of the tongue and Rathke's pouch (anterior pituitary).
TS21: tongue epithelium	Present	Regionally restricted	2C	Expression in the distal epithelium of the tongue and Rathke's pouch (anterior pituitary).
TS21: common carotid artery	Present		2C	Expression in tooth buds and carotid vessel.
TS21: tooth	Present		2C	Expression in tooth buds and carotid vessel.

Structure	Level	Pattern	Image	Note
TS23: aorta	Present	Regionally restricted	2D	Expression in abdominal aorta.

Structure	Level	Pattern	Image	Note
TS23: tooth	Present		2E	Expression in tooth buds.

Structure	Level	Pattern	Image	Note
TS23: renal arterial system	Present	Regionally restricted	2F	Expression in arcuate and interlobular arteries of the kidney.

Structure	Level	Pattern	Image	Note
TS23: urogenital sinus	Present	Regionally restricted	2G	Expression in outbuddings of the urogenital sinus.

Structure	Level	Pattern	Image	Note
TS27: palatine gland	Present		2H

Structure	Level	Pattern	Image	Note
TS27: prostate bud	Present		2I

Structure	Level	Pattern	Image	Note
TS27: tooth epithelium	Present	Regionally restricted	2J	Expression in the epithelial root sheath of the tooth.

Structure	Level	Pattern	Image	Note
TS27: hair root sheath	Present		2K

Structure	Level	Pattern	Image	Note
TS27: olfactory epithelium	Present		2L
TS27: olfactory lobe	Present		2L

Structure	Level	Pattern	Image	Note
TS27: cerebellar cortex	Present

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		2M

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		2N	Expression in external granular layer of cerebral cortex.

Structure	Level	Pattern	Image	Note
TS28: interlobular artery	Present