Antibody:	HNF-1beta antibody
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	An alternate antibody (MGI:5448186) described in paper may have been used.

Structure	Level	Pattern	Image	Note
TS15: pancreas primordium	Present	Regionally restricted	2A	Uniform expression in all Pdx1-expressing pancreatic progenitors.

Structure	Level	Pattern	Image	Note
TS15: foregut-midgut junction	Present	Regionally restricted		Expression throughout the gut epithelium at the foregut-midgut junction extending from both sides of the pancreatic bud.

Structure	Level	Pattern	Image	Note
TS15: pancreas primordium	Present	Regionally restricted	2E , 2C	Expression in the dorsal pancreatic bud.

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium	Present		2H , 2F	Expression in most pancreatic cells.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	2I , 2K	Expression in occasional cell clusters a adjacent to Hnf1b expression domain.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Regionally restricted	2L , 2N	Expression in cells in duct-like structures.

Structure	Level	Pattern	Image	Note
TS21: pancreas	Present		2W , 2U

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Regionally restricted	2Z , 2X	Expression was complementary to that of Hnf1a.

Structure	Level	Pattern	Image	Note
TS21: pancreas	Present	Regionally restricted	5V , 5T	Expression in endocrine cell precursors is downregulated.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Regionally restricted	5Y , 5W	Expression in endocrine cell precursors is downregulated.

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	7B	Expression almost exclusively in ductal cells.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present		7D

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	7F	Expression almost exclusively in ductal cells.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present		7H

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	7J	Expression almost exclusively in ductal cells.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present		7L

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	7N	Expression almost exclusively in ductal cells.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present		7P

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	7R	Expression almost exclusively in ductal cells.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present		7T