Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:180596
Ohyama K, et al., Spatio-temporal expression pattern of the NatB complex, Nat5/Mdm20 in the developing mouse brain: Implications for co-operative versus non-co-operative actions of Mdm20 and Nat5. Gene Expr Patterns. 2012 Jan;12(1-2):36-45
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:5448544
|
Gene symbol:
|
Sox1
|
Gene name:
|
SRY (sex determining region Y)-box 1
|
Antibody:
|
Anti-Sox1/3
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor
|
Assay notes:
|
The antibody used in this assay recognizes Sox1 and Sox3 (see assay MGI:5448546).
|
|
Results
|
Specimen
3A:
embryonic day 12.5
(more )
Note: Double labeled: red - Sox1 and Sox3; green - Naa20 (Nat5; assay MGI:5448638).
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Sox1 and Sox3; green - Naa20 (Nat5; assay MGI:5448638).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: ganglionic eminence |
Present |
Regionally restricted |
3A Sox1/3
,
3A Sox1/3 Nat5
|
Expression was detected in the proliferating progenitors in the ventricular zone of the ganglionic eminence. Co-expression was noted with Nat5 (Naa20).
|
Specimen
3B:
embryonic day 12.5
(more )
Note: Double labeled: red - Sox1 and Sox3; green - Naa20 (Nat5; assay MGI:5448638).
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Sox1 and Sox3; green - Naa20 (Nat5; assay MGI:5448638).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: preoptic area |
Present |
Regionally restricted |
3B Sox1/3
,
3B Sox1/3 Nat5
|
Expression was detected in the proliferating progenitors in the ventricular zone. Co-expression was noted with Nat5 (Naa20).
|
Specimen
3C:
embryonic day 12.5
(more )
Note: Double labeled: red - Sox1 and Sox3; green - Naa20 (Nat5; assay MGI:5448638).
Specimen
3C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Sox1 and Sox3; green - Naa20 (Nat5; assay MGI:5448638).
|
|