Probe:	Probe 1
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Similar results were obtained using Probe 2 (MGI:5451021).

Structure	Level	Pattern	Image	Note
TS19: jaw primordium mesenchyme	Present	Regionally restricted	2A	Expression is broadly detected in the dental epithelium and mesenchyme of developing incisors. Its level is slightly stronger in the epithelium.
TS19: oral epithelium	Present	Regionally restricted	2A	Expression is broadly detected in the dental epithelium and mesenchyme of developing incisors. Its level is slightly stronger in the epithelium.

Structure	Level	Pattern	Image	Note
TS21: incisor epithelium	Present	Widespread	2C , 2B	Expression in the epithelium is slightly stronger than that in the mesenchyme.
TS21: incisor mesenchyme	Present	Widespread	2C , 2B	Expression in the epithelium is slightly stronger than that in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: cervical loop	Strong	Regionally restricted	2D , 2E	There is strong expression in the labial cervical loop area.
TS22: incisor epithelium	Strong	Regionally restricted	2D , 2E	There is strong expression in the labial cervical loop area.
TS22: incisor mesenchyme	Present		2E , 2D

Structure	Level	Pattern	Image	Note
TS24: incisor enamel organ	Strong	Non-Uniform	2G , 2H , 2I	There is strong expression in the labial side, esp. the labial cervical loop area, including the basal epithelium, the stellate reticulum, and the outer enamel epithelium. It is also present in transit amplifying cells and the lingual cervical loop.
TS24: cervical loop	Strong	Non-Uniform	2H , 2G , 2I	There is strong expression in labial cervical loop area; there is also expression in the lingual cervical loop.
TS24: outer enamel epithelium	Strong		2I , 2H , 2G
TS24: stellate reticulum	Strong		2I , 2H , 2G
TS24: incisor mesenchyme	Present		2G

Structure	Level	Pattern	Image	Note
TS26: incisor enamel organ	Strong	Non-Uniform	2J , 2L , 2K	There is strong expression in the labial side, esp. the labial cervical loop area, including the basal epithelium, the stellate reticulum, and the outer enamel epithelium. It is also present in transit amplifying cells and the lingual cervical loop.
TS26: cervical loop	Strong	Non-Uniform	2J , 2K , 2L	There is strong expression in the labial cervical loop area; expression is also present in the lingual cervical loop.
TS26: outer enamel epithelium	Strong		2K , 2J , 2L
TS26: stellate reticulum	Strong		2K , 2J , 2L
TS26: incisor mesenchyme	Present		2J

Structure	Level	Pattern	Image	Note
TS27: incisor enamel organ	Strong	Non-Uniform	2O , 2N , 2M	There is strong expression in the labial side, esp. the labial cervical loop area, including the basal epithelium, the stellate reticulum, and the outer enamel epithelium. It is also present in transit amplifying cells and the lingual cervical loop.
TS27: cervical loop	Strong	Non-Uniform	2M , 2O , 2N	There is strong expression in the labial cervical loop area; expression is also present in the lingual cervical loop.
TS27: outer enamel epithelium	Strong		2N , 2O , 2M
TS27: stellate reticulum	Strong		2N , 2O , 2M
TS27: incisor dental papilla	Present		2M	Expression is present in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS27: inner enamel epithelium	Present	Regionally restricted	2P	Expression is present in pre-ameloblasts and ameloblasts.
TS27: molar enamel organ	Present	Regionally restricted	2P	Expression is present in pre-ameloblasts and ameloblasts.

Structure	Level	Pattern	Image	Note
TS28: molar enamel organ	Strong	Non-Uniform	2Q	In the incisors, there is strong expression in the labial side, esp. the labial cervical loop area, including the basal epithelium, the stellate reticulum, and the outer enamel epithelium. It is also present in transit amplifying cells.
TS28: cervical loop	Strong	Regionally restricted	2Q	There is strong expression in labial cervical loop area.
TS28: outer enamel epithelium	Strong		2Q
TS28: stellate reticulum	Strong		2Q

Structure	Level	Pattern	Image	Note
TS28: incisor enamel organ	Strong	Non-Uniform	2R	In the incisors, there is strong expression in the labial side, esp. the labial cervical loop area, including the basal epithelium, the stellate reticulum, and the outer enamel epithelium. It is also present in transit amplifying cells.
TS28: cervical loop	Strong	Regionally restricted	2R	There is strong expression in labial cervical loop area.
TS28: outer enamel epithelium	Strong		2R
TS28: stellate reticulum	Strong		2R

Structure	Level	Pattern	Image	Note
TS28: incisor dental pulp	Present	Regionally restricted	2S	Cells are present in the perivascular region.

Structure	Level	Pattern	Image	Note
TS26: incisor dental papilla	Present	Regionally restricted	2T	Expression is present in the odontoblasts.

Structure	Level	Pattern	Image	Note
TS27: incisor enamel organ	Present	Regionally restricted	2U	Expression is present in pre-ameloblasts and ameloblasts.
TS27: inner enamel epithelium	Present	Regionally restricted	2U	Expression is present in pre-ameloblasts and ameloblasts.
TS27: incisor dental papilla	Present	Regionally restricted	2U	Expression is present in the odontoblasts.

Structure	Level	Pattern	Image	Note
TS19: oral epithelium	Present	Regionally restricted	3A	Expression is present in the dental epithelium, including the dental lamina, of developing molars.
TS19: jaw primordium mesenchyme	Present	Regionally restricted	3A	Expression is present in the mesenchyme underlying the dental epithelium of developing molars.

Structure	Level	Pattern	Image	Note
TS21: molar epithelium	Present		3B	Expression in the dental epithelium is stronger than that in the underlying mesenchyme.
TS21: molar mesenchyme	Present		3B	Expression in the dental epithelium is stronger than that in the underlying mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: molar epithelium	Present		3C	Expression in the dental epithelium is stronger than that in the underlying mesenchyme.
TS22: molar mesenchyme	Present		3C	Expression in the dental epithelium is stronger than that in the underlying mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: molar enamel organ	Strong	Regionally restricted	3E , 3D	There is strong expression in the inner enamel epithelium.
TS24: inner enamel epithelium	Strong		3E , 3D

Structure	Level	Pattern	Image	Note
TS26: molar enamel organ	Present	Regionally restricted	3G , 3F , 3H	Expression is primarily localized in the stratum intermedium layer, the stellate reticulum adjacent to SI layer, and the inner enamel epithelium, especially in the cervical loop area.
TS26: stellate reticulum	Present	Regionally restricted	3F , 3H , 3G	Expression is seen adjacent to stratum intermedium layer.
TS26: cervical loop	Present		3G , 3H , 3F
TS26: inner enamel epithelium	Present		3G , 3H , 3F
TS26: stratum intermedium	Present		3G , 3H , 3F

Structure	Level	Pattern	Image	Note
TS27: molar enamel organ	Present	Non-Uniform	4A , 4B , 4C	Expression is primarily localized in the stratum intermedium layer, the stellate reticulum adjacent to stratum intermedium layer, and the inner enamel epithelium, esp. in the cervical loop area. It is also present in ameloblasts.
TS27: stratum intermedium	Present		4A , 4C , 4B
TS27: stellate reticulum	Present	Regionally restricted	4B , 4C , 4A	Expression is seen adjacent to the stratum intermedium layer.
TS27: inner enamel epithelium	Present		4C , 4B , 4A	Areas of expression include ameloblasts.
TS27: cervical loop	Present		4C , 4B , 4A
TS27: molar dental papilla	Present	Regionally restricted	4B , 4A	Expression is present in odontoblasts.

Structure	Level	Pattern	Image	Note
TS28: molar enamel organ	Present	Regionally restricted	4D , 4F	Expression is present in the cells of the Hertwig's epithelium root sheath.
TS28: Hertwig's epithelial root sheath	Present		4F , 4D
TS28: molar dental papilla	Present	Regionally restricted	4E , 4D	Expression is present in odontoblasts.

Structure	Level	Pattern	Image	Note
TS28: molar enamel organ	Present	Regionally restricted	4G	Expression is present in the cells of the Hertwig's epithelium root sheath.
TS28: Hertwig's epithelial root sheath	Present		4G
TS28: molar dental pulp	Present	Regionally restricted	4G	Expression is detected in cells around blood vessels.

Structure	Level	Pattern	Image	Note
TS28: molar dental pulp	Present	Regionally restricted	4I	Expression is detected in cells around blood vessels.

Structure	Level	Pattern	Image	Note
TS19: eye	Present		S1A

Structure	Level	Pattern	Image	Note
TS19: brain	Present		S1B

Structure	Level	Pattern	Image	Note
TS26: hair follicle	Present		S1C	Expression is present in the dermal papilla.