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Gene Expression Data
Assay Details
Assay
Reference: J:117808 Shih HP, et al., Expression pattern of the homeodomain transcription factor Pitx2 during muscle development. Gene Expr Patterns. 2007 Feb;7(4):441-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5461569
Gene symbol: Myod1
Gene name: myogenic differentiation 1
Antibody: anti-MyoD
Detection system: Secondary antibody coupled to Cy5
Assay notes: The authors described using two antibodies but did not specify which was used in the pictured assay; it is possible MGI:2673272 was used to generate some or all of these data.
Results
Specimen 3E: embryonic day 10.25; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS17: limb mesenchyme Present 3E Expression is present in limb muscle precursors, as shown in higher magnification in the inset.
Specimen 3H: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3H Expression is present in muscle precursors.
Specimen 3H': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3H' Expression is present in muscle precursors.
Specimen 4A: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
Structure Level Pattern Image Note
TS20: tongue muscle Present 4A
TS20: head mesenchyme Present Regionally restricted 4A Expression is present in the masseter anlagen.
Specimen 4C: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
Structure Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 4C Expression is present in the deep back muscle anlagen.
TS20: paraxial mesenchyme Present Regionally restricted 4C Expression is present in the intercostals muscle, latissimus dorsi and abdominal muscle anlagen.
TS20: limb mesenchyme Present Regionally restricted 4C Expression is present in the extensor and flexor muscle anlagen.
Specimen 4E: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 4E Expression is present in the muscle anlagen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/22/2024
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