Probe:	Gli cDNA3
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS7: embryo	Absent

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: neural fold	Present	Regionally restricted	3A Gli1	Expression in the ectoderm was confined to the ventral part of the neural fold.
TS11: embryo mesenchyme	Present	Widespread	3A Gli1
TS11: embryo endoderm	Absent		3A Gli1

Structure	Level	Pattern	Image	Note
TS11: embryo mesenchyme	Present	Widespread	3B Gli1
TS11: extraembryonic mesenchyme	Present		3B Gli1
TS11: embryo endoderm	Absent		3B Gli1

Structure	Level	Pattern	Image	Note
TS11: embryo mesenchyme	Present	Widespread	3C Gli1
TS11: embryo endoderm	Absent		3C Gli1

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	5A/B	Expression was detected in the ventral region of the developing neural tube.
TS13: somite	Present		5A/B
TS13: head paraxial mesenchyme	Strong		5A/B
TS13: head mesenchyme	Present	Regionally restricted	5A/B	Expression was detected in paraxial cephalic mesoderm, but not in head ectomesenchyme.

Structure	Level	Pattern	Image	Note
TS13: somite	Present		5C/D
TS13: splanchnopleure	Present		5C/D
TS13: trunk unsegmented mesenchyme	Present		5C/D

Structure	Level	Pattern	Image	Note
TS28: lung	Absent
TS28: cerebellum	Present	Regionally restricted		Expression was detected in Golgi cells.

Structure	Level	Pattern	Image	Note
TS28: ovary	Absent

Structure	Level	Pattern	Image	Note
TS28: testis	Absent

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	6A Gli	Expression was detected in the most anterior and ventral part of the telencephalon.
TS19: midbrain	Present	Regionally restricted	6A Gli	Expression was detected in ventral mesencephalon, including the cranial flexure.
TS19: Rathke's pouch	Present		6A Gli
TS19: axial skeleton	Present		6A Gli
TS19: gut mesenchyme	Present		6A Gli
TS19: lung mesenchyme	Present		6A Gli
TS19: myelencephalon ventricular layer	Present		6A Gli
TS19: limb	Present		6A Gli	Expression was detected in the developing limb bones.

Structure	Level	Pattern	Image	Note
TS19: axial skeleton	Present		6B Gli
TS19: limb mesenchyme	Present	Regionally restricted	6B Gli	Expression was detected in the condensing mesenchyme (blastema) of the developing limb.

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present		7A Gli
TS20: forebrain ventricular layer	Present		7A Gli
TS20: pons	Present		7A Gli
TS20: midbrain	Present	Regionally restricted	7A Gli	Expression was detected in the ventral part of the tectum.
TS20: head	Present	Regionally restricted	7A Gli	Expression was detected in superficial fronto-nasal mesenchyme and absent in central regions.
TS20: fronto-nasal process mesenchyme	Present	Regionally restricted	7A Gli	Expression was detected in superficial fronto-nasal mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: brain	Weak		7B Gli
TS22: choroid plexus	Present		7B Gli
TS22: meninges	Present		7B Gli

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	7C Gli	Expression was diminished in the brain except in the cerebellum.
TS28: cerebellum	Present		7C Gli

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted		Expression was detected in the ventral ventricular zone.
TS15: branchial arch mesenchyme derived from neural crest	Present
TS15: optic vesicle	Present
TS15: head mesenchyme derived from neural crest	Present	Regionally restricted		Expression was detected in crest-derived facial mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: gut mesenchyme	Present		8A Gli
TS22: lung mesenchyme	Present		8A Gli
TS22: vertebral cartilage condensation	Present	Regionally restricted	8A Gli	Expression was detected in the perichondrium of the vertebra.
TS22: heart	Absent		8A Gli
TS22: liver	Absent		8A Gli
TS22: perioptic mesenchyme	Present		8A Gli	Expression was detected in the optic capsule.
TS22: nasal capsule	Present		8A Gli
TS22: otic capsule	Present		8A Gli
TS22: tooth	Present		8A Gli
TS22: vibrissa epidermal component	Present		8A Gli
TS22: cranium	Present	Regionally restricted	8A Gli	Expression was detected close to the base of the skull.

Structure	Level	Pattern	Image	Note
TS22: genital tubercle	Present		8B Gli
TS22: mesonephros	Present		8B Gli
TS22: testis	Present		8B Gli

Structure	Level	Pattern	Image	Note
TS22: otic capsule	Present		8C Gli
TS22: tooth	Present		8C Gli

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present	Regionally restricted	8D Gli	Expression was detected in the outer layer of the neural retina.
TS22: nasal capsule	Present		8D Gli
TS22: optic stalk	Present		8D Gli