Probe:	Phox2b probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	2A	Expression in two ventral columns on either side of the floor plate from an abrupt rostral limit just anterior to the rhombomere1/rhombomere2 (r1/r2) boundary into the cervical spinal cord,
TS14: future spinal cord	Present	Regionally restricted	2A	Expression in two ventral columns on either side of the floor plate from an abrupt rostral limit just anterior to the rhombomere1/rhombomere2 (r1/r2) boundary into the cervical spinal cord,
TS14: rhombomere 2	Strong		2A	Expression is broader and more intense in rhombomeres 2 and 4.
TS14: rhombomere 4	Strong		2A	Expression is broader and more intense in rhombomeres 2 and 4.

Structure	Level	Pattern	Image	Note
TS17: rhombomere 1	Present	Regionally restricted	2B	Expression in a groups of cells in the dorsolateral aspect of r1 corresponding to the forming locus coeruleus.
TS17: rhombomere 2	Present	Regionally restricted	2B	Lateral stripe, sharply limited rostrally at r1/r2 boundary and caudally at r6/r7 boundary. Ventral column with r2-and r4-specific thickenings. Dorsal stripe of scattered cells from sharp limit at r3/r4 boundary into cervical spinal cord.
TS17: rhombomere 3	Present	Regionally restricted	2B	Lateral stripe, sharply limited rostrally at r1/r2 boundary and caudally at r6/r7 boundary. Ventral column with r2-and r4-specific thickenings. Dorsal stripe of scattered cells from sharp limit at r3/r4 boundary into cervical spinal cord.
TS17: rhombomere 4	Present	Regionally restricted	2B	Lateral stripe, sharply limited rostrally at r1/r2 boundary and caudally at r6/r7 boundary. Ventral column with r2-and r4-specific thickenings. Dorsal stripe of scattered cells from sharp limit at r3/r4 boundary into cervical spinal cord.
TS17: rhombomere 5	Present	Regionally restricted	2B	Lateral stripe, sharply limited rostrally at r1/r2 boundary and caudally at r6/r7 boundary. Ventral column with r2-and r4-specific thickenings. Dorsal stripe of scattered cells from sharp limit at r3/r4 boundary into cervical spinal cord.
TS17: rhombomere 6	Present	Regionally restricted	2B	Lateral stripe, sharply limited rostrally at r1/r2 boundary and caudally at r6/r7 boundary. Ventral column with r2-and r4-specific thickenings. Dorsal stripe of scattered cells from sharp limit at r3/r4 boundary into cervical spinal cord.
TS17: hindbrain	Present	Regionally restricted	2B	Expression in two ventral patches on both sides of the met-mesencephalic border, corresponding to the oculomotor (III) and trochlear (IV) motor nuclei.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	2C	Expression in cells at the rostral tips of the lateral columns. No expression in the ventral columns except in rhombomeres 4 and 5.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	2D	Expression in two ventral patches on both sides of the met-mesencephalic border.Expression in scattered cells outside of the columns, organized in ventrodorsal strings.

Structure	Level	Pattern	Image	Note
TS17: facial ganglion	Present	Regionally restricted	4	Expression in the geniculate (gVII), petrose (gIX) and nodose (gX) ganglia.
TS17: glossopharyngeal ganglion	Present	Regionally restricted	4	Expression in the geniculate (gVII), petrose (gIX) and nodose (gX) ganglia.
TS17: vagus ganglion	Present	Regionally restricted	4	Expression in the geniculate (gVII), petrose (gIX) and nodose (gX) ganglia.
TS17: dorsal root ganglion	Absent		4	No expression in other cranial or dorsal root ganglia.
TS17: enteric nervous system	Present		4	Expression in progenitors of the enteric nervous system.
TS17: sympathetic nervous system	Present		4	Expression in the primary sympathetic chain.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Absent		5B	No expression in the forming oculomotor (III) and trochlear (IV) nuclei.

Structure	Level	Pattern	Image	Note
TS17: facial ganglion	Present		8A
TS17: glossopharyngeal ganglion	Present		8A
TS17: vagus ganglion	Present		8A

Structure	Level	Pattern	Image	Note
TS17: facial ganglion	Absent		8B	No expression in the anlagen of the petrose (IX) and geniculate (VII) ganglia.
TS17: glossopharyngeal ganglion	Absent		8B	No expression in the anlagen of the petrose (IX) and geniculate (VII) ganglia.
TS17: vagus ganglion	Trace		8B	Expression in a very few cells in the Xth ganglion.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	8E	Expression in the met-mesencephalic domain in the anlagen of the oculomotor (III) and trochlear (IV) nuclei and locus coeruleus.
TS17: midbrain	Present	Regionally restricted	8E	Expression in the met-mesencephalic domain in the anlagen of the oculomotor (III) and trochlear (IV) nuclei and locus coeruleus.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Absent		8F	No expression in the anlagen of the oculomotor (III) and trochlear (IV) nuclei or locus coeruleus.
TS17: midbrain	Absent		8F	No expression in the anlagen of the oculomotor (III) and trochlear (IV) nuclei or locus coeruleus.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted		Expression in a stream of cells across the midline connecting the left and right oculomotor nuclei.