Antibody:	anti-alpha6 integrin (GoH3)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: common atrial chamber cardiac muscle	Strong		2a
TS15: outflow tract	Strong	Regionally restricted	2a	Expressed in myocardium of the distal part, bearing the endocardia1 outflow ridges.
TS15: primitive ventricle cardiac muscle	Present		2a
TS15: common atrial chamber endocardial lining	Weak		2a
TS15: primitive ventricle endocardial lining	Weak		2a
TS15: atrioventricular canal	Strong	Regionally restricted	2a	Expressed in myocardium.

Structure	Level	Pattern	Image	Note
TS19: ventricle cardiac muscle	Present	Graded		Expression gradually decreases from the subendocardial layers to the subepicardial layers.

Structure	Level	Pattern	Image	Note
TS21: ventricle cardiac muscle	Weak	Regionally restricted		Expressed in the compact layer.
TS21: interventricular septum	Weak
TS21: atrioventricular canal	Strong	Regionally restricted		Expressed in myocardium.
TS21: outflow tract	Strong	Regionally restricted		Expressed in myocardium.

Structure	Level	Pattern	Image	Note
TS23: atrium cardiac muscle	Strong		2b
TS23: trabeculae carneae	Strong	Regionally restricted	2b	Expressed in the ventricular trabecular myocardium, including the papillary muscles.
TS23: mitral valve	Strong	Regionally restricted	2b	Expressed in the tips of the atrioventricular valves.
TS23: tricuspid valve	Strong	Regionally restricted	2b	Expressed in the tips of the atrioventricular valves.
TS23: interventricular septum	Absent		2b
TS23: ventricle cardiac muscle	Absent		2b

Structure	Level	Pattern	Image	Note
TS26: atrium cardiac muscle	Strong		2c
TS26: atrium endocardial lining	Strong		2c
TS26: left ventricle endocardial lining	Strong		2c
TS26: right ventricle endocardial lining	Strong		2c
TS26: trabeculae carneae	Present		2c	Expression is diminished as compared to E15.5.
TS26: pulmonary valve	Present		2d , 2c
TS26: interventricular septum	Absent		2c
TS26: ventricle cardiac muscle	Absent		2c

Structure	Level	Pattern	Image	Note
TS21: endocardial cushion	Present	Graded	3a , 3c	Prominent at the interface between the papillary muscle and the tip of the atrioventricular cushion mysenchyme, decreasing towards the center of the cushion.
TS21: atrium cardiac muscle	Strong		3a
TS21: heart ventricle	Strong	Regionally restricted	3a	Expressed in the ventricular trabeculae.
TS21: arterial system	Present	Regionally restricted	3a	Only expressed in the coronary endothelium, the endothelium in the ascending aorta and pulmonary trunk, close to the semilunar valves.

Structure	Level	Pattern	Image	Note
TS21: atrium cardiac muscle	Strong		3e
TS21: venous system	Strong	Regionally restricted	3e	Expressed in endothelium.
TS21: cardiovascular system	Strong	Regionally restricted	3e	Expressed in capillary endothelium.
TS21: pulmonary valve	Present		3e