Gene Expression Data
Assay Details
Assay
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Reference:
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J:43464
Matise MP, et al., Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons. J Neurosci. 1997 Oct 15;17(20):7805-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5467299
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Gene symbol:
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Evx1
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Gene name:
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even-skipped homeobox 1
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Probe:
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probe 3
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Probe preparation:
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Antisense RNA
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Results
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Specimen
1a:
embryonic day 10.5; En1tm2Alj/En1 +
(more )
Note: Double labeled: purple - Evx1; blue - En1 reporter (assay MGI:5467409)
Specimen
1a:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Double labeled: purple - Evx1; blue - En1 reporter (assay MGI:5467409)
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Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Regionally restricted |
1a
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Expression was detected in the ventral intermediate zone. Expressing cells were in a cluster located dorsal to and not overlapping with En1 expressing cells.
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Specimen
not shown:
embryonic day 10.5; En1tm2Alj/En1 +
(more )
Note: Also stained for En1 reporter (assay MGI:5467409)
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Also stained for En1 reporter (assay MGI:5467409)
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Structure |
Level |
Pattern |
Image |
Note |
TS17: hindbrain |
Present |
Regionally restricted |
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The relationship of En1 to Evx1 expression was also seen in the hindbrain.
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TS17: rhombomere 1 |
Absent |
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TS17: rhombomere 2 |
Present |
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The rostral boundary of expression was at the border of rhombomeres 1 and 2.
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Specimen
not shown:
embryonic day 11.5; En1tm2Alj/En1 +
(more )
Note: Also stained for En1 reporter (assay MGI:5467409)
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Agarose
|
Note:
|
Also stained for En1 reporter (assay MGI:5467409)
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
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The relationship of En1 to Evx1 expression was also seen at E11.5.
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TS19: hindbrain |
Present |
Regionally restricted |
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The relationship of En1 to Evx1 expression was also seen at E11.5.
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Specimen
1c:
embryonic day 10.5
(more )
Note: Also stained for Lmx1b (assay MGI:5467415); both purple.
Specimen
1c:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Agarose
|
Note:
|
Also stained for Lmx1b (assay MGI:5467415); both purple.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Regionally restricted |
1c
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Expressing cells were separated from the more dorsal Lmx1b-expressing cells by a gap.
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Specimen
8a:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
|
Agarose
|
Note:
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Double labeled: purple - Evx1; blue - En1 reporter (assay MGI:5467409)
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