Gene Expression Data
Assay Details
Assay
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Reference:
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J:58583
Fukada K, et al., In situ hybridisation study of type I, II, X collagens and aggrecan mRNas in the developing condylar cartilage of fetal mouse mandible. J Anat. 1999 Oct;195(Pt 3):321-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5468296
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Gene symbol:
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Col1a2
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Gene name:
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collagen, type I, alpha 2
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Probe:
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p-alpha-2R2 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1a & 1c:
embryonic day 14.5
(more )
Specimen
1a & 1c:
(close )
Genetic Background:
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C57BL/6N
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: mandible |
Present |
Regionally restricted |
1a
,
1c
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Expression was detected in cells adjacent to the posterior edge of the ossifying mandible. Expression was also noted posteriorly in the mesenchymal cell aggregation.
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Specimen
2b:
embryonic day 15.0
(more )
Specimen
2b:
(close )
Genetic Background:
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C57BL/6N
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: mandible |
Present |
Regionally restricted |
2b
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Expression was high in periosteal osteogenic cells. High expression was detected in hypertrophic chondrocytes of the cartilage matrix.
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Specimen
3b:
embryonic day 16.0
(more )
Specimen
3b:
(close )
Genetic Background:
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C57BL/6N
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: mandible |
Present |
Regionally restricted |
3b
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Expression was detected in the periosteal osteogenic cells surrounding the cartilaginous tissue. Slight expression was noted in the fibrous cell layer to the upper hypertrophic layer but is hardly detectable in the lower half of the hypertrophic layer.
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Specimen
5b:
embryonic day 18.0
(more )
Specimen
5b:
(close )
Genetic Background:
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C57BL/6N
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: mandible |
Weak |
Regionally restricted |
5b
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The expression level in the condylar cartilage decreased, but it's localization was almost the same as at E16.0.
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Specimen
6b:
embryonic day 13.0
(more )
Specimen
6b:
(close )
Genetic Background:
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C57BL/6N
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6g:
embryonic day 14.0
(more )
Specimen
6g:
(close )
Genetic Background:
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C57BL/6N
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: tibia cartilage condensation |
Present |
Regionally restricted |
6g
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Expression was detected in the chondrocytes at the epiphysis, gradually decreased toward the depths, and was not detected in the hypertrophic chondrocytes of the diaphysis.
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