Probe:	Irx1 cDNA7
Probe preparation:	Antisense RNA
Assay notes:	At least two different probes between 0.5-1.0kb were used for analysis. The authors did not provide the details of which probe(s) were used to obtain these data but did report that the detected patterns were identical.

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent
TS9: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo mesenchyme	Present	Regionally restricted	3A	Expression is present in two symmetrical patches in the anterior region of the embryo.
TS11: extraembryonic component	Absent		3A

Structure	Level	Pattern	Image	Note
TS11: embryo mesenchyme	Present	Regionally restricted	3D	Expession is present in the mesodermal wings overlying the presumptive headfold region.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	3G	Expression is present in the dorsolateral walls.
TS13: foregut diverticulum	Present		3G	Expression is present in the anterior foregut.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Weak		3J
TS13: future spinal cord	Present	Regionally restricted	3J	Expression is present in the posterior spinal cord, where it has closed.
TS13: foregut diverticulum	Present		3J
TS13: future midbrain	Present		3J

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Strong	Non-Uniform	4A	The rostral limit of expression is at the level of the pretectum. There is strong expression in the tectum. Expression is also found in the tegmentum.
TS15: future hindbrain	Present		4A	Expression is restricted to certain rhombomeres.
TS15: head mesenchyme	Present	Regionally restricted	4A	Expression is present in the mesenchyme around the optic vesicle.
TS15: 1st branchial arch ectoderm	Present		4A
TS15: 2nd branchial arch ectoderm	Present		4A
TS15: foregut	Present		4A

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	4D	The rostral limit of expression is in the dorsalthalamic regions (the prospective domain of the zona limitans intrathalamica).
TS17: neural tube	Present	Non-Uniform	4D	Expression extends into the entire ventral spinal cord, but the strongest expression remains in the most caudal region.
TS17: apical ectodermal ridge	Weak		4D	Expression is present in the region where the apical ectodermal ridge arises.
TS17: head mesenchyme	Present		4D
TS17: hindbrain	Present		4D
TS17: midbrain	Present		4D
TS17: somite	Present		4D

Structure	Level	Pattern	Image	Note
TS19: handplate	Strong	Regionally restricted		Expression is present in the forelimb digits.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		5A
TS15: foregut	Present		5A
TS15: trigeminal preganglion	Present		5A

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		5D
TS15: branchial arch ectoderm	Present	Regionally restricted	5D	Expression is restricted to the ectoderm at the level of the hindbrain.

Structure	Level	Pattern	Image	Note
TS15: tracheal diverticulum	Present		5G
TS15: neural tube	Present	Regionally restricted	5G	Expression is present in the caudal region.
TS15: surface ectoderm	Present	Regionally restricted	5G	Expression is restricted to the ectoderm at the level of the hindbrain.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	5J	Expression is limited to the caudal region, posterior to the region of the developing hindlimb.
TS15: tracheal diverticulum	Present		5J
TS15: surface ectoderm	Present		5J

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		6A
TS15: otocyst	Present	Regionally restricted	6A	Expression is present in lateroventral region; there is no expression in the dorsal region.
TS15: head mesenchyme	Present	Regionally restricted	6A	Expression is present on the ventral side of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		6D
TS15: otocyst	Present	Regionally restricted	6D	Expression is present in lateroventral region; there is no expression in the dorsal region.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Widespread		Expression is present in the entire otic vesicle.

Structure	Level	Pattern	Image	Note
TS15: rhombomere 2	Weak	Regionally restricted	7B	Expression is present in the dorsal portion.
TS15: rhombomere 3	Weak	Regionally restricted	7B	Expression is present in the dorsal portion.
TS15: rhombomere 4	Weak	Regionally restricted	7B	Expression is present in the dorsal portion.
TS15: 1st branchial arch ectoderm	Present		7B