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Gene Expression Data
Assay Details
Assay
Reference: J:135686 Hirashima M, et al., Lymphatic vessel assembly is impaired in Aspp1-deficient mouse embryos. Dev Biol. 2008 Apr 1;316(1):149-59
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5476631
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-CD31 (2H8 MAB1398Z)
Detection system: Secondary antibody
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Either MGI:1328239 or MGI:3704881 may have been used.
Results
Specimen 3G: embryonic day 14.5 (more )
Note: Double labeled: red - Pecam1; green - Acta2 (assay MGI:5476628). The AMeX method was used to process the embryos.
Structure Level Pattern Image Note
TS22: blood vessel Present Regionally restricted 3G Expression was detected in endothelial cells.
TS22: lymphatic system Weak Regionally restricted 3G Expression was detected in endothelial cells.
Specimen 3H: embryonic day 14.5; Ppp1r13btm1Suda/Ppp1r13btm1Suda (more )
Note: Double labeled: red - Pecam1; green - Acta2 (assay MGI:5476628). The AMeX method was used to process the embryos.
Structure Level Pattern Image Note
TS22: blood vessel Present Regionally restricted 3H Expression was detected in endothelial cells. Blood vessels were relatively unaffected.
TS22: lymphatic system Weak Regionally restricted 3H Expression was detected in endothelial cells. Lymphatic vessels appear distended.
Specimen 6A: embryonic day 11.5 (more )
Note: Double labeled: red - Pecam1; green - Prox1 (assay MGI:5476686).
Structure Level Pattern Image Note
TS19: cardinal vein Present Regionally restricted 6A Expression was detected in a lymphatic endothelial cell population at the dorsolateral part of the cardinal vein.
TS19: lymphatic system Present Regionally restricted 6A Expression was detected in a lymphatic endothelial cell population at the dorsolateral part of the cardinal vein.
Specimen 6B: embryonic day 11.5; Ppp1r13btm1Suda/Ppp1r13btm1Suda (more )
Note: Double labeled: red - Pecam1; green - Prox1 (assay MGI:5476686).
Structure Level Pattern Image Note
TS19: cardinal vein Present Regionally restricted 6B Expression was detected in a lymphatic endothelial cell population at the dorsolateral part of the cardinal vein as in wild type.
TS19: lymphatic system Present Regionally restricted 6B Expression was detected in a lymphatic endothelial cell population at the dorsolateral part of the cardinal vein as in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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