Antibody:	Anti-Lyve1 (ALY7)
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Fixative: methanol/dimethyl sulfoxide.

Structure	Level	Pattern	Image	Note
TS25: lymphatic system	Present	Regionally restricted	4M	Expression was detected in lymphatic endothelial cells extended from the mesentery toward the intestine.
TS25: gut	Present	Single cells	4M	Expression was detected in single cells that may be macrophages.
TS25: gut mesentery	Present	Single cells	4M	Expression was detected in single cells that may be macrophages.

Structure	Level	Pattern	Image	Note
TS25: lymphatic system	Present	Regionally restricted	4N	The extension between the mesentery and the intestine was impaired and lymphatic vessels were distended in the mutant mesentery.
TS25: gut	Present	Single cells	4N	Expression was detected in single cells that may be macrophages.
TS25: gut mesentery	Present	Single cells	4N	Expression was detected in single cells that may be macrophages.

Structure	Level	Pattern	Image	Note
TS25: lymphatic system	Present	Regionally restricted	4O	Expression was detected in lymphatic endothelial cells on the surface of the heart.

Structure	Level	Pattern	Image	Note
TS25: lymphatic system	Present	Regionally restricted	4P	The lymphatic vessel network in the heart was disorganized and had more lymphatic islands in the mutant compared to controls.