Gene Expression Data
Assay Details
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Assay
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Reference:
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J:90448
Cha KB, et al., WNT5A signaling affects pituitary gland shape. Mech Dev. 2004 Feb;121(2):183-94
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5486158
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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Anti-Isl1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The fix was 3.7% paraformaldehyde.
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Results
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Specimen
5C:
embryonic day 12.5
(more  )
Note: Specimen was either +/+ or +/-.
Specimen
5C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen was either +/+ or +/-.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pituitary gland |
Present |
Graded |
5C
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Expression was detected in a gradient in the dorsal-ventral axis.
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pituitary gland |
Present |
Graded |
5D
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The expression pattern was intact in the mutant.
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Analysis Tools
