Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:181927
Facucho-Oliveira J, et al., Ccbe1 expression marks the cardiac and lymphatic progenitor lineages during early stages of mouse development. Int J Dev Biol. 2011;55(10-12):1007-14
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5486300
|
Gene symbol:
|
Isl1
|
Gene name:
|
ISL1 transcription factor, LIM/homeodomain
|
Probe:
|
Isl1 probe18
|
Probe preparation:
|
Antisense labelled with fluorescein RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
1I:
embryonic day 8.0
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
1I:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive heart tube |
Present |
Regionally restricted |
1I
|
Expressed in second heart field progenitors.
|
Specimen
1J:
embryonic day 8.0
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
1J:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive heart tube |
Present |
Regionally restricted |
1J
|
Expressed in second heart field progenitors.
|
Specimen
1J':
embryonic day 8.0
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
1J':
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive heart tube |
Present |
Regionally restricted |
1J'
|
Expressed in second heart field progenitors.
|
Specimen
1J":
embryonic day 8.0
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
1J":
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive heart tube |
Present |
Regionally restricted |
1J"
|
Expressed in second heart field progenitors.
|
Specimen
1J'":
embryonic day 8.0
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
1J'":
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive heart tube |
Present |
Regionally restricted |
1J'"
|
Expressed in second heart field progenitors.
|
Specimen
1M:
embryonic day 8.25
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
1M:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.25
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive heart tube |
Present |
Regionally restricted |
1M
|
Expressed in second heart field progenitors.
|
Specimen
1N:
embryonic day 8.25
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
1N:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.25
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive heart tube |
Present |
Regionally restricted |
1N
|
Expressed in second heart field progenitors.
|
Specimen
1N':
embryonic day 8.25
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
1N':
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.25
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive heart tube |
Present |
Regionally restricted |
1N'
|
Expressed in second heart field progenitors.
|
Specimen
1N":
embryonic day 8.25
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
1N":
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.25
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive heart tube |
Present |
Regionally restricted |
1N"
|
Expressed in second heart field progenitors.
|
Specimen
1N'":
embryonic day 8.25
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
1N'":
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.25
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive heart tube |
Present |
Regionally restricted |
1N'"
|
Expressed in second heart field progenitors.
|
Specimen
2C:
embryonic day 8.5
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
2C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Specimen
2D:
embryonic day 8.5
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
2D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Specimen
2D':
embryonic day 8.5
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
2D':
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: heart |
Present |
|
2D'
|
|
Specimen
2D":
embryonic day 8.5
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
2D":
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: pericardial component |
Present |
Regionally restricted |
2D"
|
Expressed in the primitive proepicardium.
|
Specimen
2D'":
embryonic day 8.5
(more )
Note: Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
Specimen
2D'":
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: purple - Isl1; light blue - Ccbe1 (assay MGI:5486167).
|
|