Antibody:	MRCD-5
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present		1A
TS15: telencephalon	Present		1A

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	1B	Expression was detected in ventral thalamus. The caudal limit was around the zona limitans intrathalamica; the ventral limit was at the alar/basal boundary. Both limits were sharply delineated. Dorsal thalamus was negative.
TS20: forebrain	Present	Regionally restricted	1B	Expression was detected in the region rostral and adjacent to the ventral thalamus.
TS20: midbrain	Present	Regionally restricted	1B	Expression was detected in a portion of the ventrocaudal midbrain. Also, weak expression was noted in the pretectum with a sharp posterior limit but a gradual anterior limit near the dorsal thalamus.
TS20: cerebellum primordium	Present		1B

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		1C
TS20: telencephalon	Present	Regionally restricted	1C	Expression was detected in the olfactory bulb.
TS20: forebrain	Present	Regionally restricted	1C	Expression was detected in the eminentia thalami.
TS20: lateral ganglionic eminence	Absent		1C
TS20: medial ganglionic eminence	Absent		1C

Structure	Level	Pattern	Image	Note
TS20: caudal ganglionic eminence	Present		1D
TS20: cerebral cortex ventricular layer	Present		1D
TS20: lateral ganglionic eminence	Absent		1D

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Absent		1F
TS20: cerebral cortex ventricular layer	Present		1F
TS20: cerebral cortex	Present	Regionally restricted	1F	Expression was most intense in the ventricular zone. Expression was reduced or absent in the more medial portions.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Absent		1G
TS20: medial ganglionic eminence	Absent		1G
TS20: cerebral cortex ventricular layer	Present		1G
TS20: cerebral cortex	Present	Regionally restricted	1G	Expression was most intense in the ventricular zone. Expression was reduced or absent in the more medial portions.

Structure	Level	Pattern	Image	Note
TS18: diencephalon	Present	Regionally restricted	3A	Expression was detected in the ventral thalamus and extended beyond the ventricular sulcus at the ventral/dorsal boundary. Positive cells extended processes from the ependymal limit to the pia mater border. The dorsal thalamus was negative.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	4B	Expression was detected in the ventrocaudal region of the midbrain, in a pair of nucleus-like domains that are presumably future trochlear nuclei.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata sulcus limitans	Present		4C
TS20: medulla oblongata	Present	Regionally restricted	4C	Anterior staining was noted.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		4D
TS20: medulla oblongata sulcus limitans	Present		4D
TS20: spinal cord sulcus limitans	Present		4D