Probe:	Elk-L cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: future brain	Present	Regionally restricted	5A	A broad domain of expression was seen in the presumptive hind- and midbrain.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	5B	Expression is in the anterior hindbrain.
TS12: future midbrain	Present		5B
TS12: rhombomere	Present		5B	Rhombomere 4.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	5C	Expression is in the anterior hindbrain.
TS13: future midbrain	Present	Regionally restricted	5C
TS13: rhombomere 4	Present	Regionally restricted	5C

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present		5D
TS13: rhombomere 2	Present		5D
TS13: rhombomere 4	Present		5D

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Strong	Regionally restricted	5E	Expression is at the dorsal midbrain.
TS14: rhombomere 1	Strong		5E
TS14: rhombomere	Present	Regionally restricted	5E	Expression is at the rhombomere boundaries.

Structure	Level	Pattern	Image	Note
TS15: neural tube roof plate	Strong		5F
TS15: branchial arch	Present		5F
TS15: limb	Present		5F
TS15: somite	Present	Regionally restricted	5F	Ventral somite.
TS15: future hindbrain	Weak		5F
TS15: telencephalon	Weak		5F
TS15: diencephalon	Weak	Regionally restricted	5F	Dorsal diencephalon.
TS15: future midbrain	Absent		5F

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Present	Regionally restricted	7A	Expression is in the distal posterior region.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	7B	Expression is stronger in the posterior region.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Present	Regionally restricted	7C	Expression is at the distal edge and in an arc orthogonal to the proximal-distal axis that was broad at the anterior edge and narrowed towards the center of the limb and in a central region.

Structure	Level	Pattern	Image	Note
TS19: footplate	Present	Regionally restricted	7D	Expression is in an arc of cells that spanned the anterior-posterior axis of the hand/footplate. (Authors did not specify hand or foot is shown)
TS19: handplate	Present	Regionally restricted	7D	Expression is in an arc of cells that spanned the anterior-posterior axis of the hand/footplate. (Authors did not specify hand or foot is shown)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	7E	Upregulation over the whole proximal forelimb with radiating rays. Expression is at the edge, less at the interdigital zone.
TS20: hand	Present	Regionally restricted	7E	Upregulation over the whole proximal forelimb with radiating rays. Expression is at the edge, less at the interdigital zone.

Structure	Level	Pattern	Image	Note
TS20: foot	Present	Regionally restricted	7F	Expression is in two oval domains on the ventral side of the limb at the base of the paddle.
TS20: forelimb	Present	Regionally restricted	7F	Expression is in two oval domains on the ventral side of the limb at the base of the paddle.
TS20: hand	Present	Regionally restricted	7F	Expression is in two oval domains on the ventral side of the limb at the base of the paddle.
TS20: hindlimb	Present	Regionally restricted	7F	Expression is in two oval domains on the ventral side of the limb at the base of the paddle.

Structure	Level	Pattern	Image	Note
TS22: foot	Present	Regionally restricted	7G	Over the entire foot/handplate and outlining the developing tendons. (Authors did not specify hand or foot is shown)
TS22: hand	Present	Regionally restricted	7G	Over the entire foot/handplate and outlining the developing tendons. (Authors did not specify hand or foot is shown)
TS22: limb digit	Present	Regionally restricted	7G	Expression is at the tips.

Structure	Level	Pattern	Image	Note
TS22: foot	Present	Regionally restricted	7H	Over the entire foot/handplate and outlining the developing tendons. (Authors did not specify hand or foot is shown)
TS22: hand	Present	Regionally restricted	7H	Over the entire foot/handplate and outlining the developing tendons. (Authors did not specify hand or foot is shown)
TS22: limb digit	Present	Regionally restricted	7H	Expression is at the tips.

Structure	Level	Pattern	Image	Note
TS23: limb digit	Present	Regionally restricted		Expression is at the tips.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		9A
TS21: spinal cord floor plate	Present		9A
TS21: spinal cord ventricular layer	Present	Non-Uniform	9A	Higher levels dorsally.