Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:39797
Smith SE, et al., Expression of heparan sulfate proteoglycan (perlecan) in the mouse blastocyst is regulated during normal and delayed implantation. Dev Biol. 1997 Apr 1;184(1):38-47
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5493274
|
Gene symbol:
|
Hspg2
|
Gene name:
|
perlecan (heparan sulfate proteoglycan 2)
|
Probe:
|
perlecan cDNA
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Visualized with:
|
Autoradiography
|
Assay notes:
|
This data was presented in Fig. 3A as silver grain counts.
|
|
Results
|
Specimen
Morulae:
embryonic day 2.5
(more )
Specimen
Morulae:
(close )
Genetic Background:
|
CF-1
|
Age:
|
embryonic day 2.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Fresh Frozen
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: compacted morula |
Absent |
|
|
No significant difference in grain densities between embryos probed with a sense or antisense probe.
|
Specimen
Unhatched Blastocysts:
embryonic day 3.5
(more )
Specimen
Unhatched Blastocysts:
(close )
Genetic Background:
|
CF-1
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Fresh Frozen
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Absent |
|
|
No significant difference in grain densities between embryos probed with a sense or antisense probe.
|
Specimen
Hatched Blastocysts:
embryonic day 4.0
(more )
Specimen
Hatched Blastocysts:
(close )
Genetic Background:
|
CF-1
|
Age:
|
embryonic day 4.0
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Fresh Frozen
|
Structure |
Level |
Pattern |
Image |
Note |
TS5: blastocyst |
Present |
|
|
Approximately sixfold increase compared with the unhatched blastocysts.
|
|