Gene Expression Data
Assay Details
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Assay
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Reference:
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J:65758
Kramer PR, et al., Midline nasal tissue influences nestin expression in nasal-placode-derived luteinizing hormone-releasing hormone neurons during development. Dev Biol. 2000 Nov 15;227(2):343-57
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5493518
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Gene symbol:
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Gnrh1
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Gene name:
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gonadotropin releasing hormone 1
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Antibody:
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anti-pro-LHRH antibody (LR-5)
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Detection system:
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Secondary antibody
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Assay notes:
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Two antibodies against the decapeptide were mentioned, but it was not specified which one was used in the assay. It is possible MGI:3043770 was used to generate these data.
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Results
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Specimen
1C:
embryonic day 11.5
(more  )
Note: Double labeled: red - Gnrh1 (LHRH); green - Nes (assay MGI:5493514).
Specimen
1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Gnrh1 (LHRH); green - Nes (assay MGI:5493514).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: nasal pit epithelium |
Present |
Regionally restricted |
1C3
,
1C
,
1C1
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Expressed in a small cluster of cells in a discrete ventro-caudal area of the invaginating placode. These cells spanned across the width of the epithelial layer.
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Specimen
not shown:
embryonic day 12.5
(more )
Note: Also stained for Nes (assay MGI:5493514).
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Also stained for Nes (assay MGI:5493514).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: nose |
Present |
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No cells had coexpression with Nestin.
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Analysis Tools