Probe:	pNJ61
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: primitive endoderm	Present	Regionally restricted	3B	Expression was restricted to a small patch in the anterior region.

Structure	Level	Pattern	Image	Note
TS13: heart	Strong	Regionally restricted	3D	Expression was detected in cardiogenic mesoderm.
TS13: notochord	Present		3D	Coexpression with Sox9 was noted.
TS13: head paraxial mesenchyme	Present		3D
TS13: head mesenchyme derived from neural crest	Weak		3D

Structure	Level	Pattern	Image	Note
TS15: trunk sclerotome	Present	Regionally restricted	3F	Expression was detected in the sclerotome of the lower trunk somites. Coexpression with Sox9 was noted.

Structure	Level	Pattern	Image	Note
TS19: vertebral pre-cartilage condensation	Present	Regionally restricted	3H	Expression was detected in the mesenchymal condensation for prevertebrae.

Structure	Level	Pattern	Image	Note
TS23: metatarsus mesenchyme	Present	Regionally restricted	3J	Expression was detected in the chondrogenic mass of the tarsals and metatarsals.
TS23: tarsus	Present	Regionally restricted	3J	Expression was detected in the chondrogenic mass of the tarsals and metatarsals.

Structure	Level	Pattern	Image	Note
TS22: vertebral cartilage condensation	Present	Regionally restricted	3L	Expression was detected in chondrocytes of the lower trunk vertebrae. Coexpression with Sox9 was noted.
TS22: intervertebral disc	Weak		3L	There was a diminished level of expression in the intervertebral tissues. Coexpression with Sox9 was noted.

Structure	Level	Pattern	Image	Note
TS24: basioccipital bone	Strong	Regionally restricted	3N	Expression was stronger in proliferating chondrocytes of the basioccipital bone. Expression was diminished in hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present
TS13: gut	Present
TS13: heart	Present
TS13: otic placode	Present
TS13: somite	Present
TS13: neural ectoderm	Present	Regionally restricted		Expression was detected in the neural tube.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present
TS15: future forebrain	Present
TS15: gut	Present
TS15: head mesenchyme	Present
TS15: heart	Present
TS15: somite	Present
TS15: central nervous system	Present	Regionally restricted		Expression was detected in the neural tube.

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present
TS17: forebrain ventricular layer	Present
TS17: gut	Present
TS17: head mesenchyme	Present
TS17: heart	Present
TS17: hindbrain ventricular layer	Present
TS17: limb mesenchyme	Present
TS17: midbrain ventricular layer	Present
TS17: notochord	Present
TS17: otocyst	Present
TS17: somite	Present
TS17: central nervous system	Present	Regionally restricted		Expression was detected in the neural tube.
TS17: respiratory system	Present	Regionally restricted		Expression was detected in larynx and bronchial cartilage.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Present	Regionally restricted		Transcripts were detected in sensory epithelium of the inner ear.
TS19: branchial arch	Present
TS19: gut	Present
TS19: head mesenchyme	Present
TS19: jaw primordium	Present
TS19: limb mesenchyme	Present
TS19: notochord	Present
TS19: somite	Present
TS19: central nervous system	Present	Regionally restricted		Expression was detected in the neural tube.
TS19: respiratory system	Present	Regionally restricted		Expression was detected in larynx and bronchial cartilage.

Structure	Level	Pattern	Image	Note
TS20: respiratory system	Present			Expression was detected in bronchopulmonary tissues, larynx, and bronchial cartilage.
TS20: cranium	Present
TS20: gut	Present
TS20: head mesenchyme	Present
TS20: heart	Present
TS20: inner ear	Present
TS20: jaw primordium	Present
TS20: limb mesenchyme	Present
TS20: notochord	Present
TS20: somite	Present
TS20: central nervous system	Present	Regionally restricted		Expression was detected in the neural tube.

Structure	Level	Pattern	Image	Note
TS21: cranium	Present	Uniform
TS21: facial bone primordium	Present
TS21: head mesenchyme	Present
TS21: heart	Present
TS21: inner ear	Present
TS21: jaw	Present
TS21: jaw skeleton	Present
TS21: limb mesenchyme	Present
TS21: notochord	Present
TS21: somite	Present
TS21: respiratory system	Present			Expression was detected in bronchopulmonary tissues, larynx, and bronchial cartilage.
TS21: central nervous system	Present	Regionally restricted		Expression was detected in the neural tube.

Structure	Level	Pattern	Image	Note
TS22: cranium	Present
TS22: head mesenchyme	Present
TS22: inner ear	Present
TS22: jaw	Present
TS22: larynx	Present
TS22: limb mesenchyme	Present
TS22: somite	Present
TS22: central nervous system	Present	Regionally restricted		Expression was detected in the neural tube.

Structure	Level	Pattern	Image	Note
TS23: cranium	Present
TS23: head mesenchyme	Present
TS23: inner ear	Present
TS23: jaw	Present
TS23: larynx	Present
TS23: limb mesenchyme	Present
TS23: limb skeleton	Present
TS23: main bronchus cartilage	Present
TS23: vertebra	Present
TS23: central nervous system	Present	Regionally restricted		Expression was detected in the neural tube.

Structure	Level	Pattern	Image	Note
TS24: cranium	Present
TS24: forebrain ventricular layer	Present
TS24: head mesenchyme	Present
TS24: hindbrain ventricular layer	Present
TS24: inner ear	Present
TS24: jaw	Present
TS24: larynx	Present
TS24: limb mesenchyme	Present
TS24: limb skeleton	Present
TS24: main bronchus cartilage	Present
TS24: midbrain ventricular layer	Present
TS24: vertebra	Present
TS24: central nervous system	Present	Regionally restricted		Expression was detected in the neural tube.

Structure	Level	Pattern	Image	Note
TS25: cranium	Present
TS25: head mesenchyme	Present
TS25: jaw	Present
TS25: larynx	Present
TS25: limb mesenchyme	Present
TS25: limb skeleton	Present
TS25: main bronchus cartilage	Present
TS25: skeleton	Present
TS25: vertebra	Present
TS25: inner ear epithelium	Present	Regionally restricted		Transcripts were detected in sensory epithelium of the inner ear.

Structure	Level	Pattern	Image	Note
TS26: cranium	Present
TS26: head mesenchyme	Present
TS26: jaw	Present
TS26: larynx	Present
TS26: limb mesenchyme	Present
TS26: limb skeleton	Present
TS26: main bronchus cartilage	Present
TS26: skeleton	Present
TS26: vertebra	Present

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak		6B
TS15: sclerotome	Strong		6B
TS15: notochord	Present		6B

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Present		6D

Structure	Level	Pattern	Image	Note
TS19: hindbrain roof plate	Present		6F
TS19: hindbrain ventricular layer	Present		6F

Structure	Level	Pattern	Image	Note
TS19: ventricle cardiac muscle	Present	Regionally restricted	6H	Expression was detected in the ventricular epimyocardium.
TS19: interatrial septum	Present	Regionally restricted	6H	Expression was detected in the interatrial region epimyocardium.
TS19: branchial arch mesenchyme	Strong		6H	Expression was detected adjacent to the arch artery.
TS19: branchial pouch	Present		6H	Expression was detected adjacent to the arch artery.

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Absent
TS11: primitive endoderm	Present	Regionally restricted		Expression was detected in visceral endoderm over cardiogenic mesoderm.

Structure	Level	Pattern	Image	Note
TS19: intestine	Absent			Expression was absent from the intestine mucosa.
TS19: umbilical cord	Absent

Structure	Level	Pattern	Image	Note
TS20: bronchus epithelium	Present
TS20: lung mesenchyme	Absent
TS20: umbilical cord	Absent
TS20: urogenital ridge	Absent			Expression was absent in genital ridge mesenchyme.
TS20: metanephros	Ambiguous	Regionally restricted		The table indicated staining in metanephric tubules as +/-.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present	Regionally restricted		Expression was detected in islets but not ductal epithelium.
TS22: genital tubercle	Absent			Expression was absent in genital tubercle mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: intestine mucosa	Absent
TS24: intestine	Present	Regionally restricted		Expression was detected in the intestine submucosa.

Structure	Level	Pattern	Image	Note
TS25: cornea	Present