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Gene Expression Data
Assay Details
Assay
Reference: J:86839 Liguori GL, et al., Anterior neural plate regionalization in cripto null mutant mouse embryos in the absence of node and primitive streak. Dev Biol. 2003 Dec 15;264(2):537-49
Assay type: RNA in situ
MGI Accession ID: MGI:5504414
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Probe: Fgf8 cDNA13
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 5C: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted 5C Expression was detected in the anterior neural ridge.
TS13: foregut diverticulum Present 5C
TS13: midbrain-hindbrain junction Present 5C
TS13: embryo Present Regionally restricted 5C Expression was detected in the tail bud.
Specimen 5C': embryonic day 8.5; Criptotm1Eda/Criptotm1Eda (more )
Structure Level Pattern Image Note
TS13: embryo Present Regionally restricted 5C' Expression was detected in a circumferential ring in the proximal region of the mutant embryo. No expression was detected at the distal embryonic tip.
Specimen Table 1: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted Expression was detected in the anterior prosencephalon.
TS13: midbrain-hindbrain junction Present
Specimen Table 1: embryonic day 8.5; Criptotm1Eda/Criptotm1Eda (more )
Structure Level Pattern Image Note
TS13: future prosencephalon Absent
TS13: midbrain-hindbrain junction Present
Specimen 7B: embryonic day 8.5 (more )
Note: Double labeled: blue - Fgf8; red - Wnt1 (assay MGI:5504434).
Structure Level Pattern Image Note
TS13: midbrain-hindbrain junction Present Regionally restricted 7B Expression was detected in a narrow stripe in a region corresponding to the isthmic organizer. Staining was adjacent to Wnt1 expression.
Specimen 7B': embryonic day 8.5; Criptotm1Eda/Criptotm1Eda (more )
Note: Double labeled: blue - Fgf8; red - Wnt1 (assay MGI:5504434).
Structure Level Pattern Image Note
TS13: embryo Present Regionally restricted 7B' Staining in a narrow band adjacent to Wnt1 expression in the proximal embryo was maintained in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory