Probe:	Alpha2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	2E	Expression is on the ventricular side of the entire anterior neural tube.
TS19: cerebellum primordium	Present		2E

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	2F	Expression is on the ventricular side of the entire anterior neural tube.
TS19: diencephalon	Present	Uniform	2F

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present		2G	Expression is weaker in the superficial cortical regions.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present		2H	Expression is weaker in the superficial cortical regions.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Strong	Regionally restricted	3E	Expression is at the pial surface.
TS22: cerebral cortex subventricular zone	Weak		3E
TS22: cerebral cortex ventricular layer	Weak		3E
TS22: thalamus	Present	Regionally restricted	3E	Expression is in the caudal part.
TS22: cerebellum ventricular layer	Present		3E
TS22: epithalamus	Present		3E
TS22: inferior colliculus	Present	Regionally restricted	3E	Expression is in the mantle of the inferior colliculus.
TS22: midbrain ventricular layer	Present		3E
TS22: pretectum	Absent		3E

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Uniform	3F	Including the preoptic area.
TS22: hypothalamus	Present	Regionally restricted	3F	Expression is in the infundibular and paraventricular region of the hypothalamus.
TS22: thalamus	Present	Regionally restricted	3F	Expression is in the caudal part.
TS22: epithalamus	Present		3F
TS22: pretectum	Absent		3F

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Weak	Uniform	3G
TS22: cerebral cortex ventricular layer	Weak	Uniform	3G

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Present	Uniform	3H

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	4E	Expression is in the preoptic area.
TS25: hypothalamus	Present		4E

Structure	Level	Pattern	Image	Note
TS25: thalamus	Strong		4F
TS25: midbrain lateral wall	Present	Regionally restricted	4F	Expression is in the subventricular zone.
TS25: telencephalon	Present	Regionally restricted	4F	Expression is in the preoptic area.
TS25: cerebellum	Present		4F
TS25: hypothalamus	Present		4F
TS25: oculomotor nerve	Present		4F
TS25: diencephalon	Weak	Regionally restricted	4F	Expression is in the prethalamus.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex intermediate zone	Present		4G	Uniform expression aside from strong expression as a thin layer at the marginal zone/cortical plate boundary and also at the cortical plate/intermediate zone boundary.
TS25: cerebral cortex marginal zone	Present		4G	Uniform expression aside from strong expression as a thin layer at the marginal zone/cortical plate boundary and also at the cortical plate/intermediate zone boundary.
TS25: cortical plate	Present		4G	Uniform expression aside from strong expression as a thin layer at the marginal zone/cortical plate boundary and also at the cortical plate/intermediate zone boundary.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex subventricular zone	Present		4H
TS25: cerebral cortex ventricular zone	Present		4H

Structure	Level	Pattern	Image	Note
TS28: cortical layer VI	Strong		5J	Expression is in a set of neurons localized in the deepest part of cortical layer 6.
TS28: main olfactory bulb	Present		5J	Expression is in the rostral migratory stream and in the different layers.
TS28: rostral migratory stream	Present		5J	Expression is in the rostral migratory stream and in the different layers.
TS28: claustrum	Weak		5J
TS28: corpus striatum	Weak		5J
TS28: main olfactory bulb glomerular layer	Weak		5J
TS28: telencephalon septum	Weak		5J

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5K
TS28: cerebellum internal granule cell layer	Present		5K

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5L
TS28: cerebellum external granule cell layer	Present		5L
TS28: cerebellum internal granule cell layer	Present		5L
TS28: cerebellum molecular layer	Present		5L
TS28: deep cerebellar nucleus	Present		5L

Structure	Level	Pattern	Image	Note
TS28: cortical layer VI	Strong	Regionally restricted	5M	Expression is in a set of neurons localized in the deepest part of cortical layer 6.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong	Uniform	5N

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5O