Antibody:	anti-doublecortin (C-18, sc-8066)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Sections were post-fixed in Zamboni-mix (2% PFA/0.1% picric acid/0.025% glutaraldehyde/0.1M phosphate buffer, pH7.4).

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Absent		1A

Structure	Level	Pattern	Image	Note
TS16: telencephalon lateral wall	Weak	Regionally restricted	2A	Expression is in the frontolateral part of the lateral ventricle wall, absent in the rest of the cortical layers.

Structure	Level	Pattern	Image	Note
TS16: telencephalon	Present		2H	Expression is in the outer border of the preplate.

Structure	Level	Pattern	Image	Note
TS16: dorsal root ganglion	Present		3D

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted		Expression is in the dorsal telencephalon, particularly in the preplate, in many fibers with a tangential orientation.

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted	4A	Expression is absent in the medial part of the telencephalon (absence of expression is shown in Figure 4A).

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Single cells	4E	Expression is in a mitotic cell in the cortical plate.

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present		4G	Expression is in the frontolateral part of the lateral ventricle wall.

Structure	Level	Pattern	Image	Note
TS18: dorsal root ganglion	Present	Regionally restricted	4K	Expression is in ganglia in the middle and lower regions and in fiber tracts absent in mitotic cells.

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Absent		4M

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		5A
TS21: dorsal root ganglion	Present		5A
TS21: hypothalamus	Present		5A
TS21: pons	Present		5A
TS21: telencephalon	Present		5A
TS21: thalamus	Present		5A
TS21: spinal cord	Present	Regionally restricted	5A	Expression is in the upper parts of the spinal cord.
TS21: midbrain	Present	Regionally restricted	5A	Expression is in the tectum.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex intermediate zone	Present		5D	Expression is in tangential fibers.
TS21: cerebral cortex marginal layer	Present		5D	Expression is in tangential fibers.
TS21: cortical preplate	Present		5D	Expression is in tangential fibers.
TS21: telencephalon ventricular layer	Absent		5D

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	5I	Expression is in both radially and tangentially orientated fibers.
TS21: pons	Present	Regionally restricted	5I	Expression is in both radially and tangentially orientated fibers.

Structure	Level	Pattern	Image	Note
TS21: optic stalk	Present	Regionally restricted	5K	Expression is in fibers entering the optic vesicle through the eye stalk.

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Absent

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Strong		6A	Expression is in the upper part of the spinal cord in dorsoventral fibers in association with dorsal root ganglia.
TS21: dorsal root ganglion	Present		6A

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		6C

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex marginal layer	Present	Regionally restricted	7A	Expression is in fibers.
TS23: cortical plate	Present	Regionally restricted	7A	Expression is in fibers.
TS23: cortical subplate	Present	Regionally restricted	7A	Expression is in fibers.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted		Expression is in radial fibers.

Structure	Level	Pattern	Image	Note
TS25: tongue	Present		7D

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present		7F

Structure	Level	Pattern	Image	Note
TS25: spinal cord	Present
TS25: corpus striatum	Weak