Antibody:	anti-DCL
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Fixative was methanol/acetone/water (40:40:20). Sections were post-fixed in Zamboni-mix (2% PFA/0.1% picric acid/0.025% glutaraldehyde/0.1M phosphate buffer, pH7.4).

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	1B	Expression is in the ventricular zone, the inner wall of the third ventricle, and neocortical neuroepithelium.
TS14: future diencephalon	Present	Regionally restricted	1B	Expression is in dorsal and ventral diencephalon including the thalamus and hypothalamus.
TS14: optic vesicle	Weak		1B

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	1D	Expression is in the ventricular zone and preplate and in a radial fibers crossing the intermediate zone.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	1E	Expression is in mitotic cells in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	1F	Expression is in mitotic cells in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present		1G	Expression is in mitotic cells in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present		1I

Structure	Level	Pattern	Image	Note
TS16: telencephalon	Present	Regionally restricted	2B	Expression is in the lateral and medial parts.

Structure	Level	Pattern	Image	Note
TS16: telencephalon	Present	Regionally restricted	2E	Expression is in the preplate and in radial fibers in the intermediate zone.
TS16: telencephalon ventricular layer	Present		2E

Structure	Level	Pattern	Image	Note
TS16: telencephalon ventricular layer	Present		2F
TS16: telencephalon	Present	Regionally restricted	2F	Expression is in the preplate and in radial fibers in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS16: telencephalon ventricular layer	Present		2G
TS16: telencephalon	Present	Regionally restricted	2G	Expression is in the preplate and in radial fibers in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS16: diencephalon lateral wall ventricular layer	Present	Regionally restricted	3A	Expression is mitotic cells in the dorsal diencephalon.
TS16: diencephalon	Present	Regionally restricted	3A	Expression is in radial fibers traversing into the intermediate zone in the dorsal diencephalon.

Structure	Level	Pattern	Image	Note
TS16: diencephalon lateral wall ventricular layer	Present	Regionally restricted	3B	Expression is mitotic cells in the dorsal diencephalon.
TS16: diencephalon	Present	Regionally restricted	3B	Expression is in radial fibers traversing into the intermediate zone in the dorsal diencephalon.

Structure	Level	Pattern	Image	Note
TS16: diencephalon	Present	Single cells	3C	Expression is in an occasional mitotic cell in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS16: dorsal root ganglion	Present	Regionally restricted	3E	Expression is in fibers.
TS16: future spinal cord	Present	Regionally restricted	3E	Expression is in the inner wall of the dorsal spinal cord and in fibers traversing towards the dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted	4B	Expression is in the medial part of the lateral ventricle wall in pial-oriented radial fibers extending from the ventricular zone toward the marginal zone and in mitotic cells.

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted	4C	Expression is in the medial part of the lateral ventricle wall in pial-oriented radial fibers extending from the ventricular zone toward the marginal zone and in mitotic cells.

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted	4D	Expression is in the medial part of the lateral ventricle wall in pial-oriented radial fibers extending from the ventricular zone toward the marginal zone and in mitotic cells.

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted	4F	Expression is in the medial part of the lateral ventricle wall in pial-oriented radial fibers extending from the ventricular zone toward the marginal zone and in mitotic cells.

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Weak		4H	Expression is in the frontolateral part.

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Present	Regionally restricted	4J	In the caudal regions expression is in fibers, absent in cellular profiles and apoptotic cells.

Structure	Level	Pattern	Image	Note
TS18: dorsal root ganglion	Present		4L , 4N
TS18: future spinal cord	Present	Regionally restricted	4L , 4N	Expression is in rostrocaudally orientated fibers.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		5B
TS21: dorsal root ganglion	Present		5B
TS21: hypothalamus	Present		5B
TS21: pons	Present		5B
TS21: thalamus	Present		5B
TS21: telencephalon ventricular layer	Weak		5B

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex marginal layer	Present		5E
TS21: cortical plate	Present		5E
TS21: cerebral cortex ventricular layer	Absent		5E

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Present		5G

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Strong	Regionally restricted	5H	Expression is in radial fibers oriented toward the outer layer.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	5J	Expression is in both radially and tangentially orientated fibers.
TS21: pons	Present	Regionally restricted	5J	Expression is in both radially and tangentially orientated fibers.

Structure	Level	Pattern	Image	Note
TS21: optic stalk	Present	Regionally restricted	5L	Expression is in fibers entering the optic vesicle through the eye stalk.

Structure	Level	Pattern	Image	Note
TS21: retina	Present		5L inset	Expression is at the base.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present		6B	Expression is in the lower part of the spinal cord in rostrocaudal fibers.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	6D	Expression is in the lower part of the spinal cord in rostrocaudal fibers.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Weak		7B

Structure	Level	Pattern	Image	Note
TS25: tongue	Strong	Regionally restricted	7E	Expression is in radially oriented fibers.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex intermediate zone	Weak		7G
TS25: cerebral cortex marginal zone	Weak		7G
TS25: cerebral cortex ventricular zone	Absent		7G

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Absent