Antibody:	PS-2
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The fixative was 0.2% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS16: dermomyotome	Strong		4A	Expression is strong in the most posterior dermomyotomes.
TS16: myotome	Absent		4A

Structure	Level	Pattern	Image	Note
TS16: myotome	Present	Regionally restricted	4B	At the hindlimb level, some cells in the central myotome show expression but these are intercalated with nonexpressing cells. Expression was absent dorsomedially.

Structure	Level	Pattern	Image	Note
TS16: myotome	Strong	Regionally restricted	4C	At the forelimb level, expression was strong in the intercalated myotome and hypaxially. Expression was absent dorsomedially.
TS16: dermomyotome	Present	Regionally restricted	4C	Expression was detected in the dermomyotomal lips, first appearing in the ventrolateral and then the dorsomedial lip.

Structure	Level	Pattern	Image	Note
TS16: myotome	Present	Regionally restricted	4D	Rostrally, expression was detected in the epaxial lip, intercalated myotome and hypaxial region. Expression was absent dorsomedially.
TS16: dermomyotome	Present	Regionally restricted	4D	Expression was detected in the dermomyotomal lips, first appearing in the ventrolateral and then the dorsomedial lip.

Structure	Level	Pattern	Image	Note
TS17: myotome	Strong	Regionally restricted	4E	Strong expression was detected in intercalated myotome and on cells in hypaxial myotome.
TS17: dermomyotome	Strong	Regionally restricted	4E	Strong expression was detected in the dermomyotomal lip.
TS17: somite	Strong	Regionally restricted	4E	No expression was seen between the myotomal and dermomyotomal domains.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present	Regionally restricted	4F	In caudal myotomes, expression was detected in most Myf5-positive cells in the central myotome. Only Myf5 is present epaxially.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present	Regionally restricted	4G	Expression was detected in myogenin-positive cells in the central myotome.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present	Regionally restricted	4H	Expression was absent in MyoD-positive cells dorsomedially, but was present in MyoD-positive cells aligned centrally.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present	Regionally restricted	5A	Expression was detected in differentiated, elongated cells. Less mature caudal myotomes also have expression in a medial position near the basal lamina.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present	Regionally restricted	5B , 5C	Expression on some elongated myocytes in the intercalated myotome extend their distal tips between the cells of the dermomyotome and appear to contact myocytes from the adjacent segment.

Structure	Level	Pattern	Image	Note
TS19: paraxial mesenchyme	Present	Regionally restricted	5D	Expression was detected between adjacent somites, colocalizing with fibronectin.